FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1854890
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC23710812.782860439163507No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1071835.7784019537546705No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC509502.7467936104027735No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGACACGATGACCAGGGTTCC476782.5703950099466817No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC359141.936179503905892No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC325071.7525028438344052No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC318591.7175681576805095No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC312131.6827412946320268No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC310491.6738997999881395No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC301631.6261341642900657No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGACACGATGACCAGGGTTCC211351.1394206664546147No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC197321.0637827580072134No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC183230.9878213802435724No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC144320.7780515286620769No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAATTCC136990.7385343605281176No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC115190.6210071756276653No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT72230.3894031451999849No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC71900.3876240639606661No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC71050.3830415819806026No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCTGGGTTCC67490.3638490692170425No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAATTCC67090.3616926071087774No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC63430.3419609788181509No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC59030.3182398956272339No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC58070.31306438656739755No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC54850.2957048665958628No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGACACGCTGACCAGGGTTCC51560.27796796575538174No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC50000.26955776353314753No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC48440.2611475613109133No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG47660.2569424601997962No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCCGGGAACC47620.2567268139889697No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC44040.23742647811999634No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC43770.23597086619691734No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGTGGTCCC40530.21850352311996937No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC40270.21710182274959702No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGTTACCAGGGTTCC38190.20588821978661806No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAATAACCGTGGTCCC37290.20103618004302143No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC36230.19532155545611868No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC35890.19348856266409328No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC30930.16674843252160507No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCTGGGTTCC29860.1609798963819957No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGATTCC28970.15618176819110569No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGTTCC28010.15100625913126922No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC27090.14604639628225932No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC27060.14588466162413943No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC26460.14264996846174166No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAAGAGACGGTGACCTTTGTCCC26020.14027786014264998No Hit
AAGACCGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGCTCC25190.13580320126799972No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGGAGAGACAGTGACCATTGTCCC25160.13564146660987983No Hit
AAGACCGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC25080.13521017418822678No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCCGGGTTCC24860.13402412002868094No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGACACGCTGACCAGGGTTCC23170.12491306762126056No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGTGGTCCC23000.12399657122524786No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCCGGGAACC22640.12205575532780918No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTGCC22450.12103143582638323No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC22340.12043840874661031No Hit
AAGACCGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC21180.1141846686326413No Hit
AAGAGCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC21060.11353773000016175No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC20740.1118125603135496No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGAAGACGGCGACCAGGGTCCC20710.1116508256554297No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA20190.10884742491468496No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG20040.10803875162408554No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGATGACCAGAGTCCC19910.10733790143889935No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC19900.10728398988619271No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC19740.10642140504288664No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGACGACCGCGGTCCC19200.10351018119672864No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAGACACGGTGACCAGTGTCCC18810.1014076306411701No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)