FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001892513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001892513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4375282
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA317480.7256217999205536Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA299720.6850301306292943Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC282790.6463354819186512Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT240760.5502731023965998Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA160090.3658964153624841Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT145320.33213859129537254Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG136220.3113399319175313Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG129360.2956609425403894Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG97040.221791418244584Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG86110.19681017132152853Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC84230.1925133054280844Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC81880.18714222306127926Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC77180.17640005832766892Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG71190.1627095122097273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC69710.15932687310212232Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT62910.14378501774285635Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA62610.14309934765347698Clontech Universal Primer Mix Long (96% over 25bp)
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG60210.1376139869384419No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA56570.12929452318730542Clontech SMART CDS Primer II A (96% over 26bp)
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA53910.12321491506147489No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC53370.12198070890059201No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG52880.12086078108793902No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC52160.11921517287342849Clontech Universal Primer Mix Long (96% over 26bp)
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT48510.11087285345264603No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG47020.10746735867539509Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG45810.10470182264823158No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC45740.10454183296070972No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA44370.10141060621921055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)