FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001893210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001893210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560718
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC72840.4667082714494226No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT55480.35547741488212475No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA35280.22604980528192795No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC35200.22553722068945192No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT34880.2234868823195478No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA33290.21329926354408676No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC29170.18690115703157137No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC26290.16844811170243437No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC25440.1630019004073766No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT25300.16210487737054355No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA24440.15659459300142625No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT23950.1534550123725106No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT23800.15249391626161804No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA23080.1478806549293338No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC23020.1474962164849768No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG22540.14442070893012063No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC22450.1438440512635851No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC22300.14288295515269253No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT21600.1383978399685273No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC21100.13519418626555213No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG19960.12788985582276874No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA19950.12782578274870923No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC19390.12423769060137706No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG19280.12353288678672253No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC17870.11449858334433255No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA17660.11315304878908297No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC17130.1097571758639293No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC16600.1063613029387756No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG16600.1063613029387756No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACAT16050.10283728386550293No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC15870.10168396853243186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)