FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001893814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001893814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436214
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAGGTACAGGAATATTAACCTGTTTCCCATCAGCTACGCATCTCT85690.5966381054633919No Hit
ATATTTATATAGATGCATGGTAGGAGAGCGTTCTGTAGGCCTGCGAAGGT66090.4601681922053399No Hit
ATATAAATATAGAGCATCCGAAGCTTCGGTTACGTGCTTAGCCCCGTTAC58650.4083653271726916No Hit
CCTTTATACAATGCGATGTGGGGACGGAGAAGGTTAGGTCAGCCGGGTGT44410.30921575754031083No Hit
AGTCATAACAGTGCTCTACCCCCGAGAATGATATACGAGGCACTACCTAA40490.2819217748887004No Hit
GTTATGACTAGGGGGATCATAAGATCTTACCAACTCATTGCAAACTCCGA39280.27349684657021867No Hit
GTCTTATACAACTGAAGCTTAGTGGCTTTTCTTGGAAGCTTGGTATCAAT37630.2620083079541071No Hit
GTATAAGACCGTACCGCAAACCGACACAGGTGGGTAGGATGAGAATTCTA37620.2619386804473428No Hit
TATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTAGCT32620.22712492706518667No Hit
GTACATGGGGGATCAAGTGAATAAGTGCATATGGTGGATGCCTAGGCGAT31530.21953552882787664No Hit
ACCTAAATAGTTTTCGGAGAGAACCAGCTATTTCCAAGTTTGTTTAGCCT29660.20651518506295025No Hit
GTAGGAGAGCGTTCTGTAGGCCTGCGAAGGTGTTCTGTGAGGGATGCTGG28960.20164125958944834No Hit
ATCCACAGCTCATCCCCAAATTTTTCAACATTTGTGGGTTCGGACCTCCA26740.18618395308777103No Hit
CCTTTTGTATAATGGGTCAGCGACTTACATTCAGTAGCAAGCTTAACCGA26560.1849306579660134No Hit
CCCTATATATTCAGGAGGAGTTACCCTTGCGGGTGGGTTTCCCCATTCGG26520.18465214793895618No Hit
CCTTAGGGGCCGACTCACCCTGCGCCGATGAACGTTGCGCAGGAAACCTT24740.17225845173490859No Hit
ATCCATGGCCAGGATGAAGGTGCCGTAACAGGTACTGGAGGTCCGAACCC24550.17093552910638665No Hit
ATATTAACCTGTTTCCCATCAGCTACGCATCTCTGCCTCACCTTAGGGGC23020.16028252057144687No Hit
GTATAATGGGTCAGCGACTTACATTCAGTAGCAAGCTTAACCGATAGGGG22510.15673151772646693No Hit
GCTCTATATTTATATAGATGCATGGTAGGAGAGCGTTCTGTAGGCCTGCG22190.15450343751000895No Hit
GTATAGACTTGCTCTAGAAGCACGATAGGGTTGCAGTGAAAAGGTGGCTG21510.14976876705003572No Hit
GTTTGTATGCATACGGTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTC21430.1492117469959212No Hit
ATACAAAAGGTACGCAGTCACGGAACAAGTCCGCTCCCACTGTTTGTATG20660.14385042897506917No Hit
GTGTAGACCCGAAACCAAGTGATCTATCCATGGCCAGGATGAAGGTGCCG20340.14162234875861118No Hit
CTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTATGCCTTC19500.13577363819040894No Hit
GTCTATGCCTTCCCACTTCGTTTTCCACTTAGCACGTCATTTGGGACCTT19430.13528624564305874No Hit
AGTCTATACACACTACCAGGGCGCACCTTCTCCCGAAGTTACGGTGCTAA18440.12839312247339185No Hit
CCTAAATAGTTTTCGGAGAGAACCAGCTATTTCCAAGTTTGTTTAGCCTT18370.12790572992604166No Hit
GAATATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTA18120.12616504225693384No Hit
CTCTATATTTATATAGATGCATGGTAGGAGAGCGTTCTGTAGGCCTGCGA17780.12379770702694724No Hit
ATATAGAGCATCCGAAGCTTCGGTTACGTGCTTAGCCCCGTTACATCTTC17530.12205701935783943No Hit
GTATAAGGGGCTATCACCCTCTATGGCCGGACTTTCCATTCCGTTCTACT17000.11836676149933088No Hit
ATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTAGCTC16690.1162083087896372No Hit
ATGATATACGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTATTT16450.1145372486272937No Hit
CCCTTATACGAAATCCCGTGTGTGGAACTAGGGTAACGACAAGTAGGGCG16260.11321432599877178No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACA16100.11210028589054277No Hit
TGGTAGGAGAGCGTTCTGTAGGCCTGCGAAGGTGTTCTGTGAGGGATGCT15760.10973295066055615No Hit
CATACAAACAGTGGGAGCGGACTTGTTCCGTGACTGCGTACCTTTTGTAT15500.10792263548468405No Hit
ATATAGATGCATGGTAGGAGAGCGTTCTGTAGGCCTGCGAAGGTGTTCTG15220.10597306529528329No Hit
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGT15190.10576418277499036No Hit
CTACCAGGGCGCACCTTCTCCCGAAGTTACGGTGCTAATTTGCCGAGTTC14660.1020739249164818No Hit
TCTATACACACTACCAGGGCGCACCTTCTCCCGAAGTTACGGTGCTAATT14490.1008902573014885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)