FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001893815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001893815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436214
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAGGTACAGGAATATTAACCTGTTTCCCATCAGCTACGCATCTCT86970.605550426329224No Hit
ATATTTATATAGATGCATGGTAGGAGAGCGTTCTGTAGGCCTGCGAAGGT64330.44791375101482095No Hit
ATATAAATATAGAGCATCCGAAGCTTCGGTTACGTGCTTAGCCCCGTTAC55850.38886962527868413No Hit
CCTTTATACAATGCGATGTGGGGACGGAGAAGGTTAGGTCAGCCGGGTGT43180.30065157420830046No Hit
AGTCATAACAGTGCTCTACCCCCGAGAATGATATACGAGGCACTACCTAA39680.27628194684079116No Hit
GTATAAGACCGTACCGCAAACCGACACAGGTGGGTAGGATGAGAATTCTA39010.27161690388758225No Hit
GTTATGACTAGGGGGATCATAAGATCTTACCAACTCATTGCAAACTCCGA38130.2654896832923227No Hit
GTCTTATACAACTGAAGCTTAGTGGCTTTTCTTGGAAGCTTGGTATCAAT36490.2540707721829755No Hit
GTACATGGGGGATCAAGTGAATAAGTGCATATGGTGGATGCCTAGGCGAT32730.2278908296395941No Hit
TATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTAGCT31170.21702893858436137No Hit
ACCTAAATAGTTTTCGGAGAGAACCAGCTATTTCCAAGTTTGTTTAGCCT27770.19335558628449523No Hit
ATCCACAGCTCATCCCCAAATTTTTCAACATTTGTGGGTTCGGACCTCCA27460.19119713357480153No Hit
GTAGGAGAGCGTTCTGTAGGCCTGCGAAGGTGTTCTGTGAGGGATGCTGG26870.1870891106757071No Hit
CCCTATATATTCAGGAGGAGTTACCCTTGCGGGTGGGTTTCCCCATTCGG26260.18284183276308405No Hit
ATCCATGGCCAGGATGAAGGTGCCGTAACAGGTACTGGAGGTCCGAACCC26170.18221518520220525No Hit
CCTTAGGGGCCGACTCACCCTGCGCCGATGAACGTTGCGCAGGAAACCTT26130.181936675175148No Hit
CCTTTTGTATAATGGGTCAGCGACTTACATTCAGTAGCAAGCTTAACCGA24630.17149254916050113No Hit
GTTTGTATGCATACGGTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTC23620.16446017097730561No Hit
GTATAATGGGTCAGCGACTTACATTCAGTAGCAAGCTTAACCGATAGGGG23530.1638335234164268No Hit
ATATTAACCTGTTTCCCATCAGCTACGCATCTCTGCCTCACCTTAGGGGC23420.16306762084201937No Hit
GTGTAGACCCGAAACCAAGTGATCTATCCATGGCCAGGATGAAGGTGCCG23330.16244097328114054No Hit
GTATAGACTTGCTCTAGAAGCACGATAGGGTTGCAGTGAAAAGGTGGCTG21910.15255386732060822No Hit
GTCTATGCCTTCCCACTTCGTTTTCCACTTAGCACGTCATTTGGGACCTT21250.1479584518741636No Hit
ATACAAAAGGTACGCAGTCACGGAACAAGTCCGCTCCCACTGTTTGTATG21190.14754068683357774No Hit
CTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTATGCCTTC19680.1370269333121666No Hit
GCTCTATATTTATATAGATGCATGGTAGGAGAGCGTTCTGTAGGCCTGCG19680.1370269333121666No Hit
GTATAAGGGGCTATCACCCTCTATGGCCGGACTTTCCATTCCGTTCTACT18840.13117822274396435No Hit
GAATATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTA17820.12407621705400448No Hit
AGTCTATACACACTACCAGGGCGCACCTTCTCCCGAAGTTACGGTGCTAA17810.12400658954724018No Hit
CCTAAATAGTTTTCGGAGAGAACCAGCTATTTCCAAGTTTGTTTAGCCTT17690.12317105946606842No Hit
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGT17620.12268366691871825No Hit
ATATAGAGCATCCGAAGCTTCGGTTACGTGCTTAGCCCCGTTACATCTTC17320.12059484171578888No Hit
ATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTAGCTC16880.11753123141815913No Hit
CTCTATATTTATATAGATGCATGGTAGGAGAGCGTTCTGTAGGCCTGCGA16660.11599942626934426No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACA16370.1139802285731792No Hit
CCCTTATACGAAATCCCGTGTGTGGAACTAGGGTAACGACAAGTAGGGCG15860.11042922572819928No Hit
TGGTAGGAGAGCGTTCTGTAGGCCTGCGAAGGTGTTCTGTGAGGGATGCT15650.10896704808614872No Hit
ATATAGATGCATGGTAGGAGAGCGTTCTGTAGGCCTGCGAAGGTGTTCTG15620.10875816556585578No Hit
GATCTATCCATGGCCAGGATGAAGGTGCCGTAACAGGTACTGGAGGTCCG15590.10854928304556286No Hit
ATGATATACGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTATTT15330.10673896786969073No Hit
CTACCAGGGCGCACCTTCTCCCGAAGTTACGGTGCTAATTTGCCGAGTTC15220.10597306529528329No Hit
GGCATAGACAGCCAGGATGTTGGCTTAGAAGCAGCCATCATTTAAAGAAA15130.1053464177344045No Hit
CTCTATGGCCGGACTTTCCATTCCGTTCTACTATCGCATATGCTAAAAAT14560.10137764984883867No Hit
TCTATACACACTACCAGGGCGCACCTTCTCCCGAAGTTACGGTGCTAATT14380.10012435472708106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)