FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001895476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001895476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452178
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT274801.8923300036221453Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA242091.667082134559262Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGT175551.2088738432891835Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA117350.8080965281115676Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT105550.7268392717697142Clontech Universal Primer Mix Long (96% over 26bp)
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAA96560.6649322603702852Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACG93240.6420700492639332Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGG92980.6402796351411466Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA84510.5819534519872908Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTAC77040.5305134769979989Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGG74350.5119895770353221Clontech Universal Primer Mix Long (96% over 26bp)
ACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT67310.4635106715567926Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATG66270.4563490150656462Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA66070.45497177343273343Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGT63550.4376185288580326Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT62240.42859759616245396Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGC61660.4246035954270069Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA59170.4074569370972429Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT56930.39203183080861986Clontech Universal Primer Mix Long (96% over 26bp)
GCTTACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT52890.36421154982378195Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG52210.35952892827187855Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG51970.3578762383123832Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTG48470.3337745097364097Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG47690.3284032673680499Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACAT47600.3277835086332392Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC43600.30023867597498377Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGC42250.2909422949528226Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTAC42170.2903913982996575Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTAC38430.2646369797641887Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCAC38420.26456811768254307Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC36870.2538944950274691Clontech Universal Primer Mix Long (96% over 26bp)
TGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAAC35460.24418494151543405Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGC34010.2341999396768165Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAG33200.2286221110635198Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT32460.22352631702174253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC32310.22249338579705794Clontech SMART CDS Primer II A (96% over 26bp)
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA31750.21863710922490218Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCA30390.20927186612109536Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGT28810.19839165722108448Clontech Universal Primer Mix Long (100% over 23bp)
GCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAG28610.19701441558817168Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGG28390.19549944979196768Clontech Universal Primer Mix Long (96% over 26bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC28080.19336472526095286No Hit
ACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATC27890.19205634570968572Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26050.17938572268688824No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG25890.17828392938055804Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG25040.17243065244067876Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTA24310.16740372048054716Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG23910.1646492372147216Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT23830.1640983405615565No Hit
TGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATG23700.16320313350016322Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC23480.16168816770395916Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA23470.16161930562231352Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT23140.15934685692800746Clontech Universal Primer Mix Long (100% over 23bp)
GGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG22530.15514626994762348Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCA22460.15466423537610405Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC21220.14612533725204485Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTCC19720.13579602500519908Clontech SMART CDS Primer II A (96% over 26bp)
GATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGT19170.13200861051468898Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTC18400.1267062302279748Clontech Universal Primer Mix Long (96% over 26bp)
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC18260.12574216108493586No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTAC18070.12443378153366874Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA18030.12415833320708618Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC17130.11796074585897873Clontech Universal Primer Mix Long (96% over 26bp)
TTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTG16960.11679009047100286Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG15560.10714939904061349Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA15450.10639191614251145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15150.1043260536931423No Hit
CTGCTTACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC14890.1025356395703557Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA14640.10081408752921474Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAA14630.1007452254475691Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)