FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001896118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001896118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1720872
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC90760.5274070355029311No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT82280.47812969238851005No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC56720.3296003421521182No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA48300.2806716594842615No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA48270.2804973292609793No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT44100.2562654282247605No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA42120.2447596334881386No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC41570.24156357939463252No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT41070.23865807567326333No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG39140.22744283130877835No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA37030.21518160560460045No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT34290.1992594452114974No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC34040.19780669335081286No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC33480.19455252918287938No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT33340.193738988140896No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA32960.19153080531265546No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC31840.18502247697678853No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC31740.1844413762325147No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT30800.17897902923634063No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA29930.1739234527611583No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG29450.17113416918864388No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG29070.16892598636040335No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC27020.15701342110278976No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT24430.14196291182609747No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG24140.14027771966770333No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT23970.13928984840243783No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC23950.13917362825358306No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT23580.13702355549976988No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG23440.13621001445778652No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC23180.13469915252267453No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG21950.12755161336810641No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC21930.12743539321925162No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC20940.12168249585094068No Hit
TATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGAT20810.1209270648833847No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG20780.12075273466010256No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACAT20740.12052029436239302No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCC19710.11453495669637255No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA19400.11273354438912367No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC19230.11174567312385814No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19040.11064158170973785No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG19040.11064158170973785No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC18810.10930504999790804No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA18760.10901449962577112No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG18680.10854961903035205No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA18550.10779418806279607No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG17910.10407514329944353No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT17580.10215751084333988No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA17470.10151830002463867No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT17470.10151830002463867No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)