FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001896198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001896198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1731229
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAACATGTACTCTGCGTTGATACCACTGCTTAACATGTACTCTGCGT144630.8354180758293676Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGTTAAGCAGTGGTATCAACGCAGAGTACATGTTAAGCAGTGG79350.45834490988771565Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTTAAGCAGTGGTATCAACGCAGAGTACATGTTAAGCAGTGGTATCAA72400.4182000185995036Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTAACATGTACTCTGCGTTGATAC52730.30458131188883736Clontech SMART CDS Primer II A (96% over 26bp)
AACATGTACTCTGCGTTGATACCACTGCTTAACATGTACTCTGCGTTGAT51570.297880869601884Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGTTAAGCAGTGGTATCAACGCAGAGTACATGTTAAGCAGTGGTA47020.27159896235564446Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC39810.22995224779621876No Hit
GCGTTGATACCACTGCTTAACATGTACTCTGCGTTGATACCACTGCTTAA35750.20650069979188196Clontech SMART CDS Primer II A (96% over 26bp)
GTTAAGCAGTGGTATCAACGCAGAGTACATGTTAAGCAGTGGTATCAACG33290.19229114114886015Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31530.18212495285141367No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT30210.1745003116283288No Hit
TGTTAAGCAGTGGTATCAACGCAGAGTACATGTTAAGCAGTGGTATCAAC24950.14411727160300572Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGTTAAGCAGTGGTATCAACGCAGAGTACATGT24770.1430775477998578Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTAACATGTACTCTGCGTTGATACCACTGCTTAACATGTACTCTGC24000.13862984041972495Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGTTAAGCAGTGGTATCAACGCAGAGTACATG22980.13273807220188663Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGTTAAGCAGTGGTATCAACGCAGAGTACAT22170.12805931508772092Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA20870.12055019873165249No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC20470.11823970139132373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18720.10813127552738545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)