FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001900690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001900690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846182
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA66880.3622611421842484Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT58400.31632850932356615Clontech Universal Primer Mix Long (96% over 26bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC51180.2772207723832212No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT34600.18741380860608542No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG30200.16358083872554277Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT27870.1509601978569827Clontech Universal Primer Mix Long (96% over 26bp)
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC27200.1473310865342637No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26990.14619360388087416No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA25470.1379603961039594Clontech SMART CDS Primer II A (96% over 26bp)
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA25110.13601042584100592No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT23430.12691056461388964No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA23120.12523142355412412No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21100.11428992374532955Clontech Universal Primer Mix Long (96% over 25bp)
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA21040.11396492870150397No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT19890.10773585702818031No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC19760.10703170109989156Clontech Universal Primer Mix Long (96% over 26bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA19000.10291509721143419No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG18840.10204844376123265No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC18710.10134428783294387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)