FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001919603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001919603
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences9345014
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTCCCATGTCTCCCATATCTGTAAGAGG212492222.73856411558078No Hit
GATCGTGAATCTGATTGCCGCAACCAACACA3847194.1168370641285295No Hit
GATCAGTATATTCTGAGGGTTTCTCCGGATT1065321.1399875912438442No Hit
GATCCCCCATGTCTCCCATATCTGTAAGAGG1062631.1371090508799666No Hit
GATCTATGTCACTCTGCAAGGCGAAGTCGCT567240.6069974855040345No Hit
GATCACCCTGACTCCCATGTTGAGAATAAAC546890.5852211671378983No Hit
GATCATTCTTTCTGAAAGGACGGACACCATT443210.4742743028528368No Hit
GATCTGATGGCTTTATAAGGGGAAACTCCTT437910.46860282927345No Hit
GATCCCCCAAGTACAATCAGTTTCCCGAAGA421660.451213877261179No Hit
GATCCGGGTCTGGTTTCAAAACAAGCGGTGC403130.4313851215204172No Hit
GATCAGCTCACGTTAGGACAAAACAAGGAGG400910.42900952315320234No Hit
GATCATAATTATTCAGCCTTGGAAAAATAAC384670.41163127203447736No Hit
GATCAGCCCATCTCAGGAACATCCATGTATT375990.40234289643653826No Hit
GATCCAGTTGTAAACTTTGGTCAAGCCCAGA372130.39821235152777723No Hit
GATCTCTACAAGGAAAACTATGAAACACTGA369810.39572974422510226No Hit
GATCTTGGGCCAGGGAAGTGCCGGCCTGAAG365920.3915670966357033No Hit
GATCAAAGACCTTGCCCTCAAGAAATTAAAG352360.3770566849873098No Hit
GATCTAGAAGTAGAAATACCATTTGACCCAG340180.36402299664826615No Hit
GATCACTTTAAAAGCCTTTGAAATAGTTTCT326050.3489026340677499No Hit
GATCACTTTGGAGCTTTGAGTTTTGACTGCC310380.33213433388114777No Hit
GATCTCACTTAGTGTTTAGCATCAGATAAGT308950.33060410610406793No Hit
GATCATGTTTGCTCAGTTTTCCAAACTAGCA288330.30853886361218935No Hit
GATCACTCACACATTGCTGATGAGAATATAA287000.30711564477056963No Hit
GATCTGAATGTTGGCAAAACCCGGGCCAAAC285680.3057031268224959No Hit
GATCCCATCTCTACAAAAAAAATTTATAAAT284810.3047721490839928No Hit
GATCCAGCAATTCTTATTTTATTCTTCCTTT277710.29717451466632366No Hit
GATCAACATGGTTTGGTTTGGCCTAGTACAA275630.29494872880875295No Hit
GATCTAACAAAGCACCACTGGGAAAAAGTGG272870.29199528219005344No Hit
GATCACGTGAGCCAATTCTCTTTAATAAACT269340.2882178667683109No Hit
ATCTCCCATGTCTCCCATATCTGTAAGAGGG246680.26396964199304573No Hit
GATCTTTCCTGCTTTCTCTTGTGGGCATTTA243810.26089848554534No Hit
GATCATTCCCATTAGCATACCATCACATTTG234980.2514495965442106No Hit
GATCTCAAGCTGCATGCTGGGAGAACCACTA234630.25107506527010015No Hit
GATCTCGGGCGGACCCGTCCGTGTTTTGCCA234360.2507861411443579No Hit
GATCATAATATAACGTTAAGGGAAAAGCAAC234010.2504116098702474No Hit
GATCCTAGCTCAGTACAATCAGTTTCCCGAA233670.25004777948968293No Hit
GATCATTCTGATGACACATGACTTTATTCAT231590.2478219936321123No Hit
GATCTTTGTACATAAAATATCAAAGTACAAT227890.24386266302008752No Hit
GATCCCCAACCCCAGTCATTTACTCCCATAA227460.24340252459760897No Hit
GATCAAATGCGCCAAGTGCAGCATCGGCTTC225510.24131585035613642No Hit
GATCAGACAACCCGAGGATTACTAAACAAGG213740.22872089865247927No Hit
GATCCTTATTAAATCTTACTGTGCTTAGGAG212290.2271692690883074No Hit
GATCTGGTCTTTTTTCTTTTCTGAATCCTGA212020.22688034496256507No Hit
GATCACTTGCTATGTCACTGACTGATTTTGA208890.22353096528266303No Hit
GATCTCAGGATATGTCCCACGTATTACTTAT206030.22047050972850338No Hit
GATCTAAAATAGTCCTCACTCGAGTCACCAA203990.2182875274451167No Hit
GATCATTAGTGATGTTGAGCATTTTTTCATA201880.21602963890690802No Hit
GATCCACTTCTAACTTCTACAGTCAGGGCCT201590.21571931299407365No Hit
GATCTTGAGCCTCCTACGAACCTCCACCCTG197210.21103232162091998No Hit
GATCTAAAACTTACAGACATCTTCTTCACTG196220.20997293315986473No Hit
GATCACAGTCAAAAGGCATAAAAACTTTCAT194760.20841060270214684No Hit
GATCAATTCCCAGTGATTATTACACAGCAAC192610.20610991058975406No Hit
GATCCTGAAGAGTAGTATTACAAGATGGGAA192040.20549995965763132No Hit
GATCATTCCTGTAATATAGTTAACTAGAATG186310.19936834765576597No Hit
GATCTGAGAATGCATAATGTACTTTCCAACT178580.191096556944698No Hit
GATCATTAGCACTTTTATCAATGAAGTATTT177150.18956632916761815No Hit
GATCTACTGCAGCACTACCAGCTGTGGTGGG172360.18444060115907798No Hit
GATCATATGTAATGTATAACTATATATAAAC171560.18358452967539696No Hit
GATCTACTTGCAGATTTTAAAGAATTATGAA170050.181968694749949No Hit
GATCAACACTGGGATTGATTGCTAGAGCAGC169060.18090930628889373No Hit
GATCTAGTGTTTAATAGCACAGCAGGTTGAC168600.18041706518577713No Hit
GATCTAAGATTCTTTTCTCAGCTTGGTCTGT167930.1797001053181943No Hit
GATCGTGCCGCTGTACAGCCTCCCTCTCACC167170.17888683740869732No Hit
GATCACTAGACCACAATTATGTCACATGTTA165430.17702488193169105No Hit
GATCAAGTATTTCCGATGAAAATTTAGTGTC165340.17692857388977692No Hit
GATCATGTACACAGCCCCTTTTTCTAGTAAA164820.17637212742538427No Hit
GATCCAGAGTAGGGTGACTATACTTAACAAA161320.17262681468427976No Hit
GATCAGGCATCATTTCAAGACAAAAATTACA161040.1723271896649914No Hit
GATCGGGTACAATCAGTTTCCCGAAGATAAG154910.1657675419212855No Hit
GATCTTGTTCTTTTTTATGGCTGCATAGTAG154570.1654037115407211No Hit
GATCGTTTTGCTAATGATAACTATCAACAGC150890.16146578271578832No Hit
GATCACCTGAGGTCGGGAATTAGAGACCAGC149520.15999976029998458No Hit
GATCAATAGGCGGTAGTAGTATTAATAAAAA147130.1574422467424875No Hit
GATCTGAACTGCCCTCCTTAGCCTGTCCAGC147010.15731383601993534No Hit
GATCAGACATAGACAGGAAAATCTCACAGGC146690.15697140742646293No Hit
GATCCAGTGCTGTCTCTTGGTCAAAGAAGAT146630.15690720206518682No Hit
GATCTACTTTTTCTTTTCCAGTGGTCAGGAT145450.15564449662675733No Hit
GATCTCCCATGTCTCCCATACCTGTAAGAGG141610.1515353535050884No Hit
GATCTATCTCATGAAATGTAACTGTTGTAAA141250.15115012133743191No Hit
GATCTTTTCTGCTTTCTCTTGTGGGCAGTTA139710.14950218373134594No Hit
GATCTTTTGCCTATTTAAAAAATCAGATTGA135030.1444941655518119No Hit
GATCCAGTACAATCAGTTTCCCGAAGATAAG134530.14395912087451126No Hit
GATCTCCCATGTCTCCCATATCCGTAAGAGG134290.14370229942940696No Hit
GATCTTGTAATCATGTGGCGGTACAATCAGT134230.14363809406813088No Hit
GATCTCCCATGTCTCCCATATCTGTAAGGGG132710.14201155824913692No Hit
GATCAACTACATTGGCCATACTTGGGGACTA131290.1404920313656031No Hit
GATCCACTTTACCTTAAAATTAATCACCAAG130560.13971086613674416No Hit
GATCCGCGAGGGCGCGGCGCAGCCGAGCAGC130520.1396680625625601No Hit
GATCATATCATCAGCAAACAGTGACACTTTG130190.13931493307554169No Hit
GATCAAATGTAATAGACTTGTCACCAAGTGG130040.13915441967235148No Hit
GATCCACCCGCCTCGGCCTCCCAAAGTGCTG129640.13872638393051098No Hit
GATCAAGTCATCACTGGGTAAGTAAACTACA129120.1381699374661183No Hit
GATCAGAGAAGAGAGGAATAGAATTTAAGAT127890.13685372755995873No Hit
GATCCCTCATCTTTTGGAAATAATTTGAATT126810.13569803105698933No Hit
GATCATAGTGTGGATAGCACAGGCCAGGCCA125500.13429621400246164No Hit
GATCAGGAAAATGATACCAATATTTGTTTCC123360.13200622278361487No Hit
GATCGACTTCTCCTCTTGAATCTTGCCAGCA123040.13166379419014249No Hit
GATCAGGGTGAATAGCAGCCAAGCATCACAA122770.13137487006440013No Hit
GATCTCATGAGACTTATTCACTAACACAAGA122660.13125716023539397No Hit
GATCTATCAGCTTCCAGAAGGCAAAGGAAAT122640.13123575844830196No Hit
GATCTCCAGTTCACCCAATATTTATTAGGTT122110.13066861109036326No Hit
GATCTTATGACAATTTAGGAGCCTAACATTG121380.12988744586150433No Hit
GATCTCCCATGTCTCCCATATCTGTAGGAGG121260.12975903513895218No Hit
GATCTGGAACACAACAAAGATGCACACTTTC121100.12958782084221596No Hit
GATCTTATGTTTTGGGCACTTTGCTATGGGT120610.12906347705846133No Hit
GATCCCAGATGAAGGAGCAAACATCCATGTT119860.12826091004251036No Hit
GATCTAACCTCATTTTGGCATCATTTTCTCA119580.12796128502322202No Hit
GATCCCACAGAAATACAAACTACCATCAGAG118630.12694470013635079No Hit
GATCCCCCGCCTGGCTCTGGATTATCTGCTC117870.12613143222685383No Hit
GATCTGCCCACCTCAGCCTCCCAAAATGCTG117590.12583180720756545No Hit
GATCTCCCATGCCTCCCATATCTGTAAGAGG117300.12552148129473106No Hit
GATCTTCAGGGCTGTTTTCTAACTCCAGGAT114850.12289976237595793No Hit
GATCTCCCATGTCCCCCATATCTGTAAGAGG114720.12276065075985974No Hit
GATCACTGAGGAGGCTGATTGGCCTTAGGTT114400.12241822216638733No Hit
GATCCACTCTGAAAATCTCTATCTATTAATT113560.12151934710852225No Hit
GATCTGAGTTTGTGAGAAGCCAGTCCCAGGG112860.12077028456030135No Hit
GATCTAAGTTATTTCTTCCCCTCCACTAGGA112740.12064187383774921No Hit
GATCTCAATATTCTAACTTCTAAGCAACCCC112590.12048136043455902No Hit
GATCTCCTAAGCCCAATCATCCTGCCTCAGC112480.12036365060555286No Hit
GATCACATCTCAGTTTAATTTATTAGTTACA112320.12019243630881665No Hit
GATCAGACAAAGAAAAATAATAAAAAATAGA112190.1200533246927185No Hit
GATCTGACTCACTGTACTGGGATTACAGGCG111510.11932566393158962No Hit
GATCTCAGAACAGACAGACTGCCTCCTCAAG109810.11750651202876743No Hit
GATCCATTTATCTGACCTTCATATTGCAGTA109530.11720688700947905No Hit
GATCTGATAGTTTTATAAGTGTTCAACTCTT109110.11675744948054653No Hit
GATCTAGAACTAGAAATACCATTTGACCCAG109080.11672534679990848No Hit
GATCCACCCACCTCGGCCTCCCAAAGTGCTG108830.11645782446125816No Hit
GATCGTTATAGCAATTTGGAAATGCTCAAAC108610.11622240480324587No Hit
GATCCTCTTTAATCAGGGTCAATTTCACCCC108560.11616890033551582No Hit
GATCGTGTTTTCCTGACAGTGCCCTGGATTT107060.1145637663036139No Hit
GATCTTGGCAGGAAGGAATCCAGAAAGACTC105480.11287302512334385No Hit
GATCTGGGCATGCGGGTCACCTTGGGGGAAC104820.112166766149307No Hit
GATCATTTAATTTCATATTTTGTGTGAGAAA104620.11195274827838675No Hit
GATCATTATTTTTAAAATCTCAGAAAACAAA103490.11074354730768729No Hit
GATCTAGGCTCAGTTTCAGTGCCCATGTGGT103130.11035831514003083No Hit
GATCTCCCACGTCTCCCATATCTGTAAGAGG103120.1103476142464848No Hit
GATCTGATACAAAAATTAGAAAAGTGTGTAT101950.1090956097016013No Hit
GATCCCTTCCACAACACATGGGAATTATGAG101340.10844285519529454No Hit
GATCATTGTCTAGATTTTTATAAACTCAATG100670.10772589532771165No Hit
GATCTCTTAGAGAGGTGAGTCTGATAACCTT100660.10771519443416565No Hit
GATCCTTCTCCATGGGATAATGTTAGACTCT100200.10722295333104905No Hit
GATCAGATGGTTGTAGATATATGGCATTATT100030.10704103814076685No Hit
GATCCTCCTGCCTCGGCCTTCCAAAGGGCTG99910.10691262741821467No Hit
GATCAAAATATTAAATCATTTTGGCTTTTAA99880.10688052473757663No Hit
GATCACCTGAGGTCAGGAGTTCCAGTCAGCC99180.10613146218935574No Hit
GATCCTAAAGTCAGACATGTTTTGAGATAGC98860.10578903359588332No Hit
GATCTCAGCATGTGAATTTTCAAGGGACACG98730.10564992197978516No Hit
GATCCCTAACTACTTTCTGCAACTCACGAAT98630.10554291304432503No Hit
GATCATATTGGATGTGGTACTGAGATGCACC98630.10554291304432503No Hit
GATCCAGAAGGCTTAAGAATATTTTGTTATC97880.10474034602837405No Hit
GATCTTTGCCTCCTTGGAGCTTAAAGTCTAG96880.10367025667377278No Hit
GATCATACAATTTATCTGAGTTTTGTAAAAG96560.10332782808030036No Hit
GATCCCAAGGCAGAAGAAGTTTTCTTAGTAC96200.10294259591264389No Hit
GATCCTTAAAAATCCATGGTGGAAGCAGGAT95670.10237544855470522No Hit
GATCCTCATCACAGCCCTATGAGCTGAAAAA95000.10165848868712235No Hit
GATCTATAAGCCTGCTATTACATATTTGCTT94830.10147657349684014No Hit
GATCTTAGTAGATTTACTGTTGAACACAGTT94500.10112344400982172No Hit
GATCGTACTCTTGGGGAACTCTGTTCATCTT94410.10102713596790759No Hit
GATCTCGGCTCACTGCAGCCTCCGCCTCCCC94070.10066330558734315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)