FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001963222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430331
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT25480.5921023584171254No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19880.4619699719518231No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT19650.4566252489362839No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGA18720.4350139776125819No Hit
GGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCAT18640.43315494352022044No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16280.3783134377955574No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15980.3713420599492019No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15650.36367354431821086No Hit
GGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCC15610.36274402727203015No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14440.33555565367124374No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG14070.326957620994072No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13560.3151062786552677No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTC12270.28512935391593913No Hit
GAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTCAATGTGTCTG11960.27792559680803847No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTG11580.2690951848693215No Hit
GACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCT11000.25561718769970093No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA10650.24748391354561952No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10510.24423060388398696No Hit
GCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCC10080.2342382956375441No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG9910.230287848191276No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT9650.22424598739110127RNA PCR Primer, Index 47 (95% over 22bp)
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9650.22424598739110127No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9530.22145743625255906No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG9230.21448605840620358No Hit
GGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAA9220.2142536791446584No Hit
GGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGG9200.21378892062156804No Hit
GTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAGGTG9120.21192988652920658No Hit
GTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCA8940.2077470598213933No Hit
GGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGC8850.20565564646748666No Hit
GACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTG8380.19473382117486307No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG8340.19380430412868233No Hit
GCATGGAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGT8280.19241002855941125No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT7940.18450913366687502RNA PCR Primer, Index 47 (95% over 24bp)
GCCCCAAGGACGTGCTGGTTCGCTGGCTGCAGGGGTCACAGGAGCTGCCC7800.18125582400524248No Hit
GGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGC7620.17707299729742917No Hit
CCTTCTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAG7410.17219303280498036No Hit
ATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGA7320.17010161945107372No Hit
GTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCG7200.1673130683125315No Hit
CCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTC6910.1605740697277212No Hit
GGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGCGGGGCAGC6870.15964455268154049No Hit
GGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGAC6830.15871503563535974No Hit
CCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAG6790.15778551858917902No Hit
GACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGG6650.15453220892754646No Hit
GGGGAGCATGGAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAG6470.15034938221973318No Hit
AGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGTG6420.14918748591200726No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6320.14686369329655544No Hit
GCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGA6240.14500465920419398No Hit
GGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAG6140.14268086658874216No Hit
AGCATGGAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGG5950.13826566061938367No Hit
AGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTG5770.1340828339115704No Hit
GTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGA5580.12966762794221193No Hit
GTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCG5570.12943524868066675No Hit
CCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGAAGCCG5570.12943524868066675No Hit
TCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCAT5550.12897049015757636No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5480.12734383532676008No Hit
GGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCT5400.12548480123439865No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5370.12478766344976308No Hit
GCTTGGGGGAGCATGGAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTC5320.12362576714203717No Hit
GGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAGGTGTCCCCCT5270.12246387083431126No Hit
GTCCTCGGCTGCCACGCGCAGTATGCTGGTCACAGCGAAGGTGGTGGTGC5200.12083721600349498No Hit
GTCAGGTACTTCTCGCGGGGCAGCTCCTGTGACCCCTGCAGCCAGCGAAC5090.11828104412649797No Hit
TCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAG5080.11804866486495279No Hit
CAGAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTCAATGTGTC5020.1166543892956817No Hit
GGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATG5000.11618963077259133No Hit
CCATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGC4990.11595725151104615No Hit
CAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTC4910.11409821741868469No Hit
CTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGA4910.11409821741868469No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4870.11316870037250396No Hit
GGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGCCG4840.1124715625878684No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT4820.11200680406477803No Hit
CAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTC4790.1113096662801425No Hit
GGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCA4760.11061252849550694No Hit
ACCATCGACCGCTTGGCGGGTAAACCCACCCATGTCAATGTGTCTGTTGT4700.10921825292623585No Hit
GTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTT4690.10898587366469066No Hit
CACAGAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTCAATGTG4690.10898587366469066No Hit
GTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCC4660.10828873588005511No Hit
TCACACAGAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTCAAT4620.10735921883387439No Hit
AGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTC4580.10642970178769365No Hit
CCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACC4490.10433828843378701No Hit
ACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACA4440.1031763921260611No Hit
CACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTG4440.1031763921260611No Hit
GACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGG4370.10154973729524482No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)