FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001963223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430331
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGA18510.4301340131201331No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17870.4152617403812414No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17340.4029456395193467No Hit
GGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCAT15710.36506781988748194No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14440.33555565367124374No Hit
GGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCC13370.31069107268590923No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12150.2823408027773969No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG11800.27420752862331554No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTC11340.26351808259223714No Hit
GAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTCAATGTGTCTG10920.25375815360733944No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTG10450.24283632831471588No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10440.24260394905317068No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9890.22982308966818565No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA9870.22935833114509527No Hit
GCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCC9590.22285171182183017No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG9530.22145743625255906No Hit
GACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCT9410.2186688851140169No Hit
GTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAGGTG8820.2049585086828511No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG8810.2047261294213059No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8790.20426137089821556No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG8670.20147281975967335No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8570.19914902714422153No Hit
GGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGG8270.19217764929786604No Hit
GGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGC7920.18404437514378466No Hit
GGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGC7870.18288247883605874No Hit
GTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCA7830.18195296178987802No Hit
GGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAA7660.17800251434360992No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC7420.17242541206652554No Hit
GCCCCAAGGACGTGCTGGTTCGCTGGCTGCAGGGGTCACAGGAGCTGCCC7320.17010161945107372No Hit
GACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTG7260.1687073438818026No Hit
CCTTCTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAG7100.1649892756970797No Hit
GCATGGAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGT6940.16127120751235677No Hit
GGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGAC6770.15732076006608867No Hit
GACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGG6540.15197603705054946No Hit
GTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCG6450.14988462369664282No Hit
CCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAG6280.1459341762503747No Hit
GCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGA6230.1447722799426488No Hit
GTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCG6140.14268086658874216No Hit
CCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTC5990.1391951776655644No Hit
ATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGA5720.1329209376038445No Hit
GCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAA5690.13222379981920893No Hit
GGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCT5540.12873811089603118No Hit
GGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAG5500.12780859384985047No Hit
GTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGA5470.1271114560652149No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5430.12618193901903418No Hit
CAGAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTCAATGTGTC5220.12130197452658534No Hit
GGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAGGTGTCCCCCT5200.12083721600349498No Hit
AGCATGGAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGG5140.11944294043422388No Hit
GTCCTCGGCTGCCACGCGCAGTATGCTGGTCACAGCGAAGGTGGTGGTGC5100.11851342338804316No Hit
TCACACAGAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTCAAT5090.11828104412649797No Hit
AGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGTG5070.11781628560340761No Hit
GGGGAGCATGGAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAG4990.11595725151104615No Hit
TCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAG4970.11549249298795577No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4920.11433059668022987No Hit
GCTTGGGGGAGCATGGAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTC4870.11316870037250396No Hit
AGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTG4800.11154204554168767No Hit
GGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGCGGGGCAGC4790.1113096662801425No Hit
CCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACC4770.11084490775705212No Hit
GGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGCCG4680.10875349440314548No Hit
CCATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGC4680.10875349440314548No Hit
TCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCAT4680.10875349440314548No Hit
CTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGA4610.1071268395723292No Hit
GAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAGGAGCCCAG4460.10364115064915147No Hit
ACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACA4450.10340877138760628No Hit
ACCATCGACCGCTTGGCGGGTAAACCCACCCATGTCAATGTGTCTGTTGT4450.10340877138760628No Hit
GGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATG4420.10271163360297073No Hit
GTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCC4400.10224687507988037No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)