FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001963234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2951750
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT449281.5220801219615483No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT420511.424612517997798No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT313181.0609977132209707No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284870.9650885068179894No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC258920.8771745574659101No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222690.7544338104514272No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT131980.44712458710934194No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT125320.4245617006860337No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT122630.41544846277631914Illumina PCR Primer Index 7 (95% over 22bp)
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120800.4092487507410858No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93700.3174388074870839No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC90460.30646226814601507No Hit
CTCTAACCCCACCCACGGGAGCCTGGAGCTGCAGGATCCCAGGGGAGGGG84970.28786313204031505No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC80730.2734987719149657No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA75390.2554078089269078No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG74130.25113915473871434No Hit
GTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGCCTGGAGCTGCAG71940.24371982722114No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA68550.23223511476242906No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCCCAACTCTAACC63940.21661726094689593No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG63640.21560091471161175No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCC63560.21532988904886932No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA61180.20726687558228168No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG60420.20469213178622853No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG58390.19781485559413908No Hit
CATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCC57250.1939527399000593No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTC55780.18897264334716693No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT55690.1886677394765817No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55440.18782078428051158No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA55230.18710934191581266No Hit
ACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGC55040.18646565596679934No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC52270.17708139239434234No Hit
GGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAG50990.1727449817904633No Hit
GGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGG49370.16725671211992885No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA48620.16471584653171847No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG46990.15919369865334124No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG46580.15780469213178624No Hit
CTACAGAATGTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGCCTG46110.15621241636317437No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA45570.15438299313966292No Hit
ACTCTAACCCCACCCACGGGAGCCTGGAGCTGCAGGATCCCAGGGGAGGG45450.15397645464554924No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG42030.1423901075633099No Hit
CCCCTACAGAATGTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGC41740.14140763953586855No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT41700.14127212670449735Illumina PCR Primer Index 7 (96% over 25bp)
GTCAATGAGGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGG41700.14127212670449735No Hit
GTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCCCAACTCTAA41620.1410011010417549No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAGGCAC41260.1397814855594139No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG40890.13852799186923012Illumina PCR Primer Index 7 (96% over 27bp)
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA40480.1371389853476751No Hit
GGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT39560.13402219022613704No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT39160.13266706191242483No Hit
GGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCG38590.13073600406538494No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG37240.12616244600660625No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC37170.12592529855170662No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGC37040.12548488184975015No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT37010.12538324722622174No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAAC35030.11867536207334631No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATA34550.11704920809689166No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC34510.11691369526552046No Hit
AGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGT32920.11152706021851444No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG32810.11115439993224357No Hit
GCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTAC32310.10946048954010333No Hit
GAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGG32300.10942661133226052No Hit
CCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCCCAACTCT31580.10698738036757854No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG31510.1067502329126789No Hit
GTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGG30810.10437875836368254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)