FastQCFastQC Report
Fri 10 May 2024
EGAF00001963235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2951750
Total Bases445.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC227850.7719149656983145No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT116900.3960362496823918No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100540.3406115016515626No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC82970.2810874904717541No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT72780.24656559667993566No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC70680.23945117303294655No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA69400.2351147624290675No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA67710.22938934530363345No Hit
CTCTAACCCCACCCACGGGAGCCTGGAGCTGCAGGATCCCAGGGGAGGGG66610.22566274244092488No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG65500.22190226137037353No Hit
GTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGCCTGGAGCTGCAG64580.21878546624883544No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG64130.2172609468959092No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCCCAACTCTAACC59230.2006606250529347No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCC58540.19832302871178117No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57250.1939527399000593No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA54790.18561870077072923No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG53880.18253578385703395No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG53250.18040145676293723No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTC52910.17924959769628188No Hit
CATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCC51920.17589565511984415No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA51060.1729821292453629No Hit
ACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGC51050.17294825103752012No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT50690.17172863555517914No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50280.17033962903362412No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC49880.16898450071991192No Hit
GGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAG49030.16610485305327347No Hit
GGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGG43520.14743796053188787No Hit
GTCAATGAGGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGG42540.14411789616329296No Hit
CTACAGAATGTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGCCTG42080.14255949860252393No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG41500.14059456254764122No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA41160.13944270348098586No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA40580.13747776742610315No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG40580.13747776742610315No Hit
ACTCTAACCCCACCCACGGGAGCCTGGAGCTGCAGGATCCCAGGGGAGGG40190.13615651732023376No Hit
GTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCCCAACTCTAA39400.13348013890065213No Hit
CCCCTACAGAATGTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGC38260.12961802320657237No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG38060.12894045904971627No Hit
GGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCG36140.12243584314389769No Hit
AGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGT35180.11918353519098841No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA35030.11867536207334631No Hit
GGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT34820.11796391970864742No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT34720.11762513763021935No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAGGCAC34420.11660879139493521No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGC34000.11518590666553741No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG33960.11505039383416617No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG33750.11433895146946726No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC33310.11284831032438383No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAAC33220.1125434064537986No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT32720.11084949606165835No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC32570.11034132294401626No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32520.11017193190480223No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG32320.10949436774794613No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATA31650.10722452782247818No Hit
GCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTAC31590.10702125857542137No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG30140.10210891843821462No Hit
GTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGG30100.1019734056068434No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)