FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001963268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99901
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT16311.632616290127226No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14521.4534389045154703No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13741.375361607991912No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11301.1311198086105245No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7530.7537462087466592No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.530525219967768No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT5180.5185133281949129TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT4820.4824776528763476RNA PCR Primer, Index 11 (96% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.40039639242850417No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.3483448614127987No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2850.28528242960530925No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.22922693466531865No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC2150.21521306093032103No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT2080.20820612406282218No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT1780.17817639463068438TruSeq Adapter, Index 11 (96% over 28bp)
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC1630.16316152991461547No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG1610.16115954795247295TruSeq Adapter, Index 11 (96% over 30bp)
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT1400.14013873734997648No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC1100.11010900791783866No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.10510405301248237No Hit
CAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.10310207105033985No Hit
GTGCTACACAGCTGTGGTCCCACTCGTATATGGTGGTGAGACCAAAATGG1020.10210108006926857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)