FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001963277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938454
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC77290.2630294705991654No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC75330.2563592964191374No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT74780.25448756386861937No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG68480.23304771828995793No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67230.22879378067514416No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA61300.20861310063046756No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT61290.20857906912954907No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC60320.20527801354045358No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT57320.19506856326490052No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC55330.188296294582117No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAA53560.1822727189195407No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG51470.17516013522757204No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAAGTGTTGCCG50220.17090619761275827No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC49590.1687622130548921No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT48990.1667203229997815No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA48890.16638000799059643No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47340.16110512534822735No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT46210.15725956574443567No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA46080.15681715623249504No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT45510.15487736068013996No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA45320.15423076216268827No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT45250.1539925416562587No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT44550.151610336591963No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA42580.14490613091101648No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA40600.13816789372915145No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC39490.13439039712719683No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC39370.13398201911617472No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT39030.13282494808494535No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT37680.12823069546094648No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT37490.1275840969434948No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT37340.12707362442971712No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA36440.1240107893470512No Hit
GCTCATATGTAGGCGGCAACACTTTGGTGTTCGGCGGAGGGACCAAGCTG36160.12305790732133291No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA35650.1213223007744889No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGG35370.1203694187487706No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34920.11883800120743766No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC34610.11778302467896383No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG34390.11703433165875662No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT34050.11587726062752726No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT34030.11580919762569024No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAATTATGTCTCCT33410.11369924456874261No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA33280.11325683505680198No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG32950.11213379552649115No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG32260.10978562196311394No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG30580.10406832980880422No Hit
TATGTAGGCGGCAACACTTTGGTGTTCGGCGGAGGGACCAAGCTGACCGT30460.1036599517977821No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC30320.10318351078492297No Hit
GATTATTACTGCAGCTCATATGTAGGCGGCAACACTTTGGTGTTCGGCGG30190.10274110127298233No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC29750.10124371523256787No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGA29750.10124371523256787No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29710.10110758922889385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)