FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001963279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261180
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT112460.4973509406593018No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC99080.43817829628777893No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC84590.3740967105670491No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA83380.36874552224944496No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC78880.34884440867157857No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT76160.3368152911311793No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA71960.318240918458504No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT66450.2938731104998275No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG65430.2893621914221778No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT63140.27923473584588576No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC61210.27069936935582306No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA58980.2608372619605693No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGCTTATGTCTTCGG55880.24712760594026129No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA55250.24434145003936No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT52320.23138361386532696No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC49710.21984096799016442No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCC49210.21762973314817927No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGT47810.21143827559062084No Hit
GTGCAGCATGGGATGACAGCCTGAGTGCTTATGTCTTCGGAACTGGGACC47760.2112171521064223No Hit
GCATGGGATGACAGCCTGAGTGCTTATGTCTTCGGAACTGGGACCAAGGT46290.20471612167098593No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT46140.20405275121839042No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG44860.1983919900229084No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC44850.19834776532606868No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG44340.19609230578724385No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGCACTCAGG44060.19485401427573215No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG43580.19273122882742638No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT43490.19233320655586908No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA43150.19082956686331914No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG42970.19003352232020448No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC42470.18782228747821933No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG42110.18623019839199No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC41710.1844612105184019No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG41600.18397473885316515No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG39670.17543937236310245No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC39210.1734050363084761No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCA38800.17159182373804827No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC38790.17154759904120856No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG38420.16991128525813956No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC37750.16694823056987945No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG37390.16535614148365013No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC37280.16486966981841342No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC36800.16274688437010765No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG36380.1608894471028401No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGCACTCAGGC35840.15850131347349614No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA35730.15801484180825942No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA35620.15752837014302268No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT35400.1565554268125492No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATT35360.1563785280251904No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGC35180.15558248348207573No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC35150.15544980939155661No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC34740.1536365968211288No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT34650.15323857454957146No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT34160.151071564404426No Hit
GGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG34150.1510273397075863No Hit
GGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGGAAT34090.15076199152654807No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGA33200.1468259935078145No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC32780.14496855624054697No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA31350.13864442459246942No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT31180.13789260474619447No Hit
GGATGACAGCCTGAGTGCTTATGTCTTCGGAACTGGGACCAAGGTCACCG31140.13771570595883564No Hit
GTGCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC31050.1373176836872783No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT30850.13643318975048427No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAGGAATAATCAGCGGCCCT30820.13630051565996515No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTG30460.13470842657373586No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC30390.1343988536958579No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG30220.13364703384958296No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTC29850.13201072006651396No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC29130.12882654189405532No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC29110.1287380925003759No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC28830.12749980098886424No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATACATAATTACTT28110.12431562281640561No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG28110.12431562281640561No Hit
AGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAG28030.12396182524168797No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG27900.12338690418277182No Hit
AGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGA27660.12232551145861895No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG27570.12192748918706162No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTC27500.12161791630918371No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG26690.11803571586516774No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC26610.11768191829045012No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA26470.11706277253469427No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGTGG26420.11684164905049577No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCC25740.11383436966539594No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25690.11361324618119743No Hit
TTACTGTGCAGCATGGGATGACAGCCTGAGTGCTTATGTCTTCGGAACTG25430.11246340406336514No Hit
CTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGC25230.11157891012657108No Hit
TGGGATGACAGCCTGAGTGCTTATGTCTTCGGAACTGGGACCAAGGTCAC25200.11144623603605197No Hit
TCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGG24850.10989837164666237No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC24820.10976569755614324No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG24650.1090138777098683No Hit
CTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACC24120.10666996877736402No Hit
GTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGG23920.10578547484056997No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA23800.10525477847849352No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAAC23530.10406071166382154No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG23440.10366268939226422No Hit
TGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGA23380.103397341211226No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23240.10277819545547015No Hit
GGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACT22650.10016893834192767No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)