FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001963282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776643
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT686933.8664492528887346No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT624633.5157879213775645No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421452.3721704360414555No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360802.0307962826521706No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185191.0423591008435573No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150670.8480600773481223No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT84470.47544723391249677No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83990.47274550936794846No Hit
GTACTTGTTTGCTATCGGTCTCTCACCTATATTTAGCCTTAGATGGAATT66580.3747517087000596No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT40890.23015315963871188No Hit
CACCTATATTTAGCCTTAGATGGAATTTACCACCCACTTTGAGCTGCATT39970.2249748542616609No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37360.21028422705067928No Hit
CCTATATTTAGCCTTAGATGGAATTTACCACCCACTTTGAGCTGCATTCC33720.189796149254521No Hit
TTATGATACAGTCTCTAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAAGT33710.1897398633265096No Hit
CAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33390.18793871363014403No Hit
GTCTCTAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAAGTGGGTGGTAAA28900.16266633195301475No Hit
CGGTACTTGTTTGCTATCGGTCTCTCACCTATATTTAGCCTTAGATGGAA28800.1621034726729005No Hit
GTTTGGGAATGCAGCTCAAAGTGGGTGGTAAATTCCATCTAAGGCTAAAT27730.1560808783756782No Hit
TCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26660.15005828407845584No Hit
GTGCTGGACCATGTATAAGTCTGTTGAAAAGCAGCATCATTGAGGGTGAT24440.13756280805991974No Hit
GTCTCTCACCTATATTTAGCCTTAGATGGAATTTACCACCCACTTTGAGC23140.13024563741843465No Hit
GTGCATTATGATACAGTCTCTAAGAGTCGAGTTGTTTGGGAATGCAGCTC20170.11352871679904178No Hit
ATATTTAGCCTTAGATGGAATTTACCACCCACTTTGAGCTGCATTCCCAA19560.11009527519034493No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19170.10790012399789942No Hit
AACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18890.10632411801357955No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT17810.10024523778834576RNA PCR Primer, Index 41 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)