FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001963290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113177
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT83597.385776261961352No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT23072.038400028274296Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT20611.8210413776650731Illumina PCR Primer Index 10 (95% over 23bp)
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16021.4154819442112796No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11691.03289537626903No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10920.9648603514848423No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7810.69006953709676No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT5390.47624517348931317Illumina PCR Primer Index 10 (96% over 26bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG5170.4568065949795453Illumina PCR Primer Index 10 (96% over 28bp)
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.4285322989653375No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC4800.4241144402131175No Hit
TCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG3350.29599653639873824No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.2942293928978503No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT2970.26242080988186645No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG2870.2535850923774265Illumina PCR Primer Index 10 (95% over 24bp)
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACACTCGCT2810.24828366187476256No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT2310.2041050743525628No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC2290.2023379308516748No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC2250.19880364384989882No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC2140.18908435459501488No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC2120.18731721109412688No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2120.18731721109412688No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT2090.18466649584279493No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA2080.18378292409235092No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC2030.17936506534013097No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAACCTCGTATGCCGT1930.170529347835691No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.16346077383213903No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA1820.16081005858080705Illumina PCR Primer Index 10 (96% over 28bp)
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG1810.15992648683036306No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA1650.14578933882325915No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA1580.13960433657015117No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT1550.1369536213188192No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT1540.13607004956837518No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC1540.13607004956837518No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT1530.13518647781793122No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAAGCTCGTATGCCGT1530.13518647781793122No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG1530.13518647781793122No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC1510.13341933431704323No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT1450.12811790381437926No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT1410.12458361681260326No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAAACTCGTATGCCGT1400.12370004506215929No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG1330.11751504280905131No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA1290.11398075580727532No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC1280.11309718405683135No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG1250.11044646880549935No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC1210.10691218180372337No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC1190.10514503830283538No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT1180.10426146655239138No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT1170.10337789480194738No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA1170.10337789480194738No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT1160.10249432305150338No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)