FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001963299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7084996
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT452570.6387724142681238No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC420550.5935783167696919No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC394180.5563588179866298No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA380030.5363870353631816No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC340180.48014141433530805No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC338350.4775584912115688No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT329180.46461564692485363No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA291010.41074123401057666No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG289010.40791836720867586No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG285880.4035005806637012No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG270570.38189153529515046No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT265420.3746226532802559No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG263150.3714186994600985No Hit
GCTTAAGAGTTACAGGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA262230.3701201807312241No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC258940.3654765648420973No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT257260.36310535672850064No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG245180.34605524124501974No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCG228720.3228230474653761No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC226750.32004252366550384No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC225750.31863109026455344No Hit
GTAAGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAG216540.30563178864180024No Hit
CTCTTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT215600.3043050412449068No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT208710.2945802651123586No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC206500.2914609972962582No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAAGAGACAGAGTCACCATCACTT202710.28611166470665617No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG198980.2808470181211112No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC194090.2739451087904637No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC189050.26683148444967364No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG187750.26499662102843813No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA178180.2514892033813428No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG173830.24534946808720853No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT171970.2427242019614408No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT164500.23218079445634127No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT163720.23107987640359995No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA159510.22513774178559873No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAAGT159260.22478488343536115No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA157440.22221607464563142No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT155410.21935086484170208No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA154590.21819348945292275No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT154460.2180100031107992No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC152020.21456610561248024No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC151380.21366278823587198No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC151250.21347930189374842No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGA149200.2105858634218001No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA148630.20978134638325835No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG148440.20951317403707778No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGT148350.20938614503099223No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT148130.20907562968278315No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC144650.20416384144747576No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG143940.203161723732801No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA141460.19966136889844396No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA141200.19929439621419687No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA141030.19905445253603532No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC139910.19747364712697085No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT138690.19575169837781134No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT138070.1948766096692221No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC136750.19301351757996757No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA135310.190981053482599No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC134660.19006362177198124No Hit
GTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTG134630.19002127876995273No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT132520.18704315429394738No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC130750.18454491717426516No Hit
GTCTCTTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGT129250.18242776707283956No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT128250.18101633367188916No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA124980.17640094645078136No Hit
ACTTATTACTGTCAACAGCTTAAGAGTTACAGGCTCACTTTCGGCGGAGG124910.17630214611271483No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG123230.17393093799911813No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC123150.1738180233270421No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG120990.17076932718098925No Hit
ACTGTCAACAGCTTAAGAGTTACAGGCTCACTTTCGGCGGAGGGACCAAG120460.1700212674784855No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA117970.16650679831011903No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCC117760.16621039729591944No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG114730.16193375409103972No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC111700.15765711088616No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT111180.15692316551766577No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGC110960.1566126501694567No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA107240.1513621179179212No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGT106640.15051525787735096No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG106580.15043057187329392No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT106160.14983776984489475No Hit
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCA103980.1467608450308229No Hit
GATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATG103590.14621038600445224No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCA102080.14407912156901712No Hit
AGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAA100620.14201842880362953No Hit
GAGTTACAGGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA100320.14159499878334442No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGA99200.14001419337427995No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATC97090.1370360688982746No Hit
ATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATAC96380.13603395118359982No Hit
GTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTG95670.13503183346892503No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG95510.13480600412477298No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC94690.13364862873599365No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC93820.1324206816771668No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCCTGTAACTCTTAAGCTGTTGACAG93370.13178553664673912No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA93070.13136210662645398No Hit
GGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCC92460.13050113225187424No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC92040.1299083302234751No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGT91940.12976718688338001No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAG91550.12921672785700938No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT90790.1281440384722871No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC90390.1275794651119069No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC90100.1271701494256313No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCCTGTAACTCTTAAGCTGTTGACA90000.12702900608553624No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA89580.1264362040571371No Hit
GCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCTTACAGATGCAG89350.1261115743749185No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG89190.1258857450307664No Hit
GCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAACTGCTA88860.12541997200845278No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT88560.12499654198816768No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT88300.12462956930392055No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCT88090.12433316828972098No Hit
GCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTG88040.12426259661967347No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG87350.12328870757301769No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC87340.12327459323900818No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT86780.12248419053447596No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT85820.12112921446956358No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC85530.12071989878328795No Hit
GGCTAAATAACTGCTAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTC85450.12060698411121192No Hit
GTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCA84770.11964720939856563No Hit
GTCTGCATCTGTAAGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG84600.11940726572040408No Hit
CACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAAC84250.11891326403007145No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCC83830.11832046200167226No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA83770.11823577599761526No Hit
ATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATT83460.11779823164332062No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT83290.11755828796515903No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAGTTATTTAGCC83000.11714897227888343No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC82870.11696548593675987No Hit
CACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAGTTATTTAGCCTGGT82660.1166690849225603No Hit
GGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCC82030.11577988187996154No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG81600.11517296551755286No Hit
CCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCA80950.11425553380693511No Hit
GCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCCT80800.11404381879679254No Hit
GTTACAGGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACT79670.1124488990537186No Hit
GTCAACAGCTTAAGAGTTACAGGCTCACTTTCGGCGGAGGGACCAAGGTG79500.11220895537555702No Hit
CAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGT79140.11170083935121487No Hit
GGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGCTTAGGGGCT79030.11154558167711032No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT78240.11043054929035952No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCT77770.10976717559191282No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT76670.10821459885086739No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC76550.10804522684275335No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC76550.10804522684275335No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT75840.10704310912807855No Hit
GGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACAC75460.1065067644357174No Hit
CTGTAAGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGC75450.10649265010170789No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTC75220.10616802041948929No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC75020.10588573373929921No Hit
ATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT74380.10498241636269096No Hit
CCATCCTTCCTGTCTGCATCTGTAAGAGACAGAGTCACCATCACTTGCCG73990.1044319573363203No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA73840.10422024232617776No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT73430.10364155463178809No Hit
GGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTA73240.1033733822856075No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG73100.10317578160947444No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC72230.1019478345506476No Hit
GCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCT70940.10012708546342157No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)