FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001963336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3611231
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG195420.5411451109053949No Hit
CCTCTGAGGAGCTTCAAGGCAACAAGGCCACACTGGTGTGTCTCATAAGT190460.5274101822896403No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC179350.4966450498458836No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC168270.4659629915671415No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC158470.43882543099569093No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA155010.42924421063066864No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC149880.4150385284131644No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147150.4074787793968317No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT145780.40368505919449627No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACTGTTGCTG144400.3998636476038226No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGGCAACAAGGCCACACTGGTGT141030.39053164973384424No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG139010.38493798928952483No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT132970.368212390733243No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC131320.3636433116574376No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA129750.3592957636883378No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCT128080.354671301835856No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT125020.34619773700436224No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC123420.34176711487024786No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA122200.33838876549298563No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGGCAACAAGGCCACACTG120670.33415198307723876No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG119270.33027518870988865No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT117640.3257614924107597No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA113200.31346651598859226No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA106850.2958824843938258No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT105580.2923656780748725No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT102650.2842521012917756No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT101880.282119864389733No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATA101860.2820644816130566No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100310.2777723164206333No Hit
GGCAGCAACAGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGG98170.27184635931625534No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG97270.269354134365816No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT96850.268191096055611No Hit
CACCAGTGTGGCCTTGTTGCCTTGAAGCTCCTCAGAGGAGGGCGGGAACA95910.2655881055518187No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT94550.26182207673782154No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGT92960.25741914599204535No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA90050.24936095198562483No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA87120.2412473752025279No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT86700.24008433689232284No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG85820.23764749471855995No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGCCTTGAAGCTCCTCAGAGGA85510.2367890616800753No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC85350.23634599946666385No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT85170.235847554476576No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG84560.23415837978794488No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC83290.2306415734689916No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC81580.22590634606315685No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCAACAGTGTGGTATTCGGCGG79920.2213095755990132No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA77220.21383290074769518No Hit
ACAGTACCCAGGCAAAGCCCCCAAAGTCATGATTTATGAGGTCAGTAAGC76410.2115898982922998No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG75840.21001148915702153No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT75420.2088484508468165No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA75040.2077961780899643No Hit
GTTGCTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGCCT74370.20594085507130394No Hit
GTGTGGCCTTGTTGCCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG73150.20256250569404172No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAACAGTGTGG70890.19630425192960518No Hit
ATACCACACTGTTGCTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCA70620.19555658444447335No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG70510.195251979172753No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC69790.19325819921240153No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT69770.1932028164357251No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGACTTTGGGGGCTTTG69760.19317512504738688No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA68440.18951986178674252No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68060.18846758902989036No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT67310.18639073490452426No Hit
GGGTACTGTTGGTGCCAGGAGACATAGTTATAACCACCAACGTCACTGCT63790.17664336620947263No Hit
GGTTATAACTATGTCTCCTGGCACCAACAGTACCCAGGCAAAGCCCCCAA62980.17440036375407722No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC62880.17412344987069506No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACT62760.17379115321063648No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACTGTTGCTGC62580.17329270822054862No Hit
CAACAGTACCCAGGCAAAGCCCCCAAAGTCATGATTTATGAGGTCAGTAA62220.17229581824037288No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG61990.17165891630859395No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC61720.17091124882346215No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCC61360.1699143588432864No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC61220.16952667940655142No Hit
TCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTT60990.16888977747477246No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC60760.1682528755429935No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA60500.16753289944619992No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG59770.16551142809751024No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC59590.16501298310742238No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT58850.16296382037039447No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT56740.15712093743103114No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCC56340.15601328189750255No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG56300.15590251634414967No Hit
GCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC56180.1555702196840911No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC55950.15493331775231217No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA55400.15341029139371035No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC54730.15155496837504995No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG54470.15083499227825636No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA54290.1503365472881685No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGG54270.15028116451149207No Hit
GGCACCAACAGTACCCAGGCAAAGCCCCCAAAGTCATGATTTATGAGGTC53850.14911812620128703No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA53710.14873044676455202No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC53100.1470412720759209No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGG52930.14657051847417127No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC52810.1462382218141127No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC52400.14510287489224588No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC52250.14468750406717268No Hit
GGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAACA52240.14465981267883446No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC52230.14463212129049624No Hit
GTACCCAGGCAAAGCCCCCAAAGTCATGATTTATGAGGTCAGTAAGCGGC51230.14186298245667472No Hit
CTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTG51200.1417799082916601No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA51120.1415583771849544No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC51110.14153068579661618No Hit
GTACTGTTGGTGCCAGGAGACATAGTTATAACCACCAACGTCACTGCTGG50960.14111531497154295No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGT50880.14089378386483722No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGC50090.13870616418611825No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT49930.1382631019727068No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA49600.1373492861575457No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC49380.13674007561410498No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT49350.13665700144909035No Hit
CCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTG48440.1341370851103128No Hit
GTATCAACGCAGAGTACGGGGGGGTCTCAGGAGGCAGCGCTCTCAGGACG48200.13347249179019566No Hit
GTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTA48090.13316788651847528No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC47850.13250329319835813No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG47390.13122948933480025No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGGCAACAAGGCC47390.13122948933480025No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT47270.13089719267474165No Hit
CAGCAACAGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTC46920.12992799408290415No Hit
GTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGC46890.1298449199178895No Hit
AGACACACCAGTGTGGCCTTGTTGCCTTGAAGCTCCTCAGAGGAGGGCGG44680.123725123095144No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA44630.12358666615345294No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG44540.123337443658409No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT44540.123337443658409No Hit
CTCTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCC44480.12317129532837973No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA44100.12211902257152756No Hit
GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAG43930.1216482689697779No Hit
CTGTTGCTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGC43890.12153750341642502No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC43820.12134366369805753No Hit
GGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC43440.12029139094120536No Hit
CTATGTCTCCTGGCACCAACAGTACCCAGGCAAAGCCCCCAAAGTCATGA43260.11979294595111749No Hit
GTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACT43260.11979294595111749No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT43130.11943295790272071No Hit
GCTTGGTCCCTCCGCCGAATACCACACTGTTGCTGCCTGCATATGAGCTG43020.11912835263100034No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCC42940.11890682152429462No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGCCTTGAAG42900.11879605597094176No Hit
GCTCATATGCAGGCAGCAACAGTGTGGTATTCGGCGGAGGGACCAAGCTG42680.11818684542750103No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA42510.11771609182575139No Hit
CCTGAGGGCCGCTTACTGACCTCATAAATCATGACTTTGGGGGCTTTGCC41980.116248448243826No Hit
CATATGCAGGCAGCAACAGTGTGGTATTCGGCGGAGGGACCAAGCTGACC41330.11444850800184203No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC40950.11339623524498987No Hit
CTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGC40810.11300855580825486No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG40800.11298086441991664No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG40770.11289779025490201No Hit
GTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTC40570.1123439624881377No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40190.11129168973128553No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCC39300.10882715616918441No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG39010.10802410590737617No Hit
GGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCC38510.10663953649046544No Hit
GTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGC38490.106584153713789No Hit
GGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGCACCAACA38290.10603032594702472No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT38070.10542111540358398No Hit
GCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGG37590.10409192876334966No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT37280.10323349572486501No Hit
CCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGT37150.1028735076764682No Hit
GTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTT36960.10234737129804214No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAA36910.10220891435635106No Hit
GTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATG36850.10204276602632177No Hit
CGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGG36710.10165508658958676No Hit
ATCAGGGACCCCTGAGGGCCGCTTACTGACCTCATAAATCATGACTTTGG36660.10151662964789569No Hit
GCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGCACCAACAGTACCCA36610.10137817270620463No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCC36160.10013206023098495No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)