FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001963337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3611231
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC184040.5096323109765063No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG176820.48963912859631525No Hit
CCTCTGAGGAGCTTCAAGGCAACAAGGCCACACTGGTGTGTCTCATAAGT164890.4566033023088249No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC152870.42331825352629066No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACTGTTGCTG145220.4021343414475563No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC144520.4001959442638812No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC144470.40005748732219015No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA139890.3873748314632877No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT136910.3791227977384997No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG120270.33304432754371016No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT119050.32966597816644794No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCT118590.32839217430289No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA118050.3268968393326265No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGGCAACAAGGCCACACTGGTGT117410.32512459047898074No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC114570.3172602361909277No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC113850.3152664562305762No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG110020.30466065449703994No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT109320.3027222573133649No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA109080.30205766399324774No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGGCAACAAGGCCACACTG104470.2892919339693307No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA101350.28065222080780766No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT98780.2735355340048864No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA96770.2679695649489053No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATA91710.2539577224497685No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT91330.25290544969291634No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGT88300.24451495902643722No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT87240.24157967186258644No Hit
GTTGCTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGCCT86910.24066585604742538No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT85850.2377305688835746No Hit
CACCAGTGTGGCCTTGTTGCCTTGAAGCTCCTCAGAGGAGGGCGGGAACA85530.23684444445675173No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA85170.235847554476576No Hit
GGCAGCAACAGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGG84740.23465682477803274No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT84060.23277381037103412No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT82080.22729091548006758No Hit
ATACCACACTGTTGCTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCA81420.2254632838497454No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC80620.22324797278268824No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCAACAGTGTGGTATTCGGCGG78800.21820814010513312No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG77990.21596513764973774No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGCCTTGAAGCTCCTCAGAGGA76520.21189450356402015No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG76450.21170066384565261No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT76040.21056531692378583No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA75770.20981764943865402No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC71770.1987410941033681No Hit
ACAGTACCCAGGCAAAGCCCCCAAAGTCATGATTTATGAGGTCAGTAAGC71060.19677500553135482No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC70310.1946981514059887No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG69850.19342434754243082No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT68070.18849528041822858No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA65720.1819878041587481No Hit
GGTTATAACTATGTCTCCTGGCACCAACAGTACCCAGGCAAAGCCCCCAA65680.18187703860539522No Hit
GTGTGGCCTTGTTGCCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG65390.18107398834358698No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA64670.17908020838323552No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG64490.17858176339314766No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAACAGTGTGG64280.17800024423804514No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGACTTTGGGGGCTTTG63440.17567416761763507No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT61880.17135431103687357No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA61650.17071740910509464No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACTGTTGCTGC60660.16797596165961137No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG60200.16670215779605346No Hit
GGGTACTGTTGGTGCCAGGAGACATAGTTATAACCACCAACGTCACTGCT58950.1632407342537766No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC58530.1620776959435716No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG57800.16005622459488192No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC57410.15897626044969154No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT56880.15750861686776615No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC56390.15615173883919362No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC56360.15606866467417896No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC56090.15532099718904718No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACT55150.15271800668525498No Hit
CAACAGTACCCAGGCAAAGCCCCCAAAGTCATGATTTATGAGGTCAGTAA54920.15208110475347603No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA54580.15113959754997675No Hit
CTGTTGCTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGC54030.14961657119137492No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC53620.14848122426950808No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT53540.14825969316280238No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC53500.14814892760944953No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA53440.14798277927942022No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG52880.1464320615324802No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGG52650.14579515960070125No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC52010.1440229107470555No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT51180.14172452551498368No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA51120.1415583771849544No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCC50750.14053379581644043No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG50050.1385953986327654No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG50020.13851232446775075No Hit
GTACTGTTGGTGCCAGGAGACATAGTTATAACCACCAACGTCACTGCTGG49910.13820771919603037No Hit
GGCACCAACAGTACCCAGGCAAAGCCCCCAAAGTCATGATTTATGAGGTC49690.13759850865258966No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGG49520.13712775505084002No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC49080.13590933396395855No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCC48950.13554934591556175No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC48410.13405401094529815No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA47860.13253098458669632No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT47690.13206023098494668No Hit
GTACCCAGGCAAAGCCCCCAAAGTCATGATTTATGAGGTCAGTAAGCGGC47190.13067566156803595No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC47120.13048182184966844No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA46880.1298172285295513No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC46770.12951262325783092No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC46650.12918032659777234No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT46120.12771268301584696No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC46030.127463460520803No Hit
GCTTGGTCCCTCCGCCGAATACCACACTGTTGCTGCCTGCATATGAGCTG45570.12618965665724513No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA45450.12585735999718656No Hit
GGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAACA45180.12510969251205475No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGT45110.12491585279368726No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC44960.12450048196861402No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT44890.12430664225024653No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC44880.12427895086190831No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA44650.12364204893012937No Hit
CCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTG44590.12347590060010007No Hit
CTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTG43940.12167596035811612No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC43300.11990371150447036No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG43220.11968218039776464No Hit
GTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGC42770.11843606792254498No Hit
GTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTA42620.11802069709747176No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT42620.11802069709747176No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGC42370.11732841238901637No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42240.11696842434061959No Hit
CAGCAACAGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTC40860.11314701274994594No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG40610.11245472804149055No Hit
CTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGC40180.11126399834294731No Hit
AGACACACCAGTGTGGCCTTGTTGCCTTGAAGCTCCTCAGAGGAGGGCGG40170.1112363069546091No Hit
CTCTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCC40000.11076555335285945No Hit
GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAG40000.11076555335285945No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT39690.1099071203143748No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA38980.10794103174236154No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGGCAACAAGGCC38820.1074979695289501No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC38550.1067503020438183No Hit
GTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACT38450.10647338816043615No Hit
CATATGCAGGCAGCAACAGTGTGGTATTCGGCGGAGGGACCAAGCTGACC38340.10616878288871578No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGCCTTGAAG38190.10575341206364257No Hit
CTATGTCTCCTGGCACCAACAGTACCCAGGCAAAGCCCCCAAAGTCATGA38040.10533804123856934No Hit
CCTGAGGGCCGCTTACTGACCTCATAAATCATGACTTTGGGGGCTTTGCC37880.1048949790251579No Hit
GCTCATATGCAGGCAGCAACAGTGTGGTATTCGGCGGAGGGACCAAGCTG37780.10461806514177575No Hit
GGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC37100.10273505073477715No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCC37080.10267966795810071No Hit
GTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTC36720.10168277797792498No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA36550.10121202437617532No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG36430.10087972771611675No Hit
CCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGT36390.10076896216276389No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)