FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001963344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84512
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT1343515.897150700492238No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT24672.9191120787580465Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT19642.3239303294206737Illumina PCR Primer Index 10 (95% over 23bp)
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7120.8424839076107535No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.8152688375615298No Hit
TCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG6880.814085573646346No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC6120.7241575160923892No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.6212135554714123No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT5220.6176637637258614Illumina PCR Primer Index 10 (96% over 26bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG4770.5644168875425976Illumina PCR Primer Index 10 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC4100.48513820522529344No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT3970.46975577432790616No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.4354411207875805No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACACTCGCT3360.3975766755017039No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG3330.39402688375615297Illumina PCR Primer Index 10 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAACCTCGTATGCCGT3280.38811056418023476No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAAACTCGTATGCCGT2540.3005490344566452No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAAGCTCGTATGCCGT2480.29344945096554337No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.2697841726618705No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.20588792124195382No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA1500.17748958727754638Illumina PCR Primer Index 10 (96% over 28bp)
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACACTCGCTAA1400.16565694812570997No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATATCGTATGCCGT1090.12897576675501704No Hit
GTATCAACGCAGAGTACGGGAATGCCCCAACTAAATACTACCGTATGGCC1040.12305944717909882No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT990.1171431276031806No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT910.10767701628171147No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.10649375236652782No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA880.10412722453616054No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC870.10294396062097691No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)