FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001963345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84512
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG18502.1890382430897386No Hit
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC6700.7927868231730405No Hit
CATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGT3140.37154486936766373Illumina RNA PCR Primer (100% over 25bp)
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.30173229837182886No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.2792502839833397No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.23191972737599395No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.1502745172283226No Hit
GTATCAACGCAGAGTACGGGAATGCCCCAACTAAATACTACCGTATGGCC1150.1360753502461189No Hit
TATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGT1000.11832639151836426No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)