FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001963346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584764
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136850.863535516960254No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT135690.8562158150992829No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125530.7921053229376741No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70650.4458077038600069No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT60580.38226511960140436No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT46840.295564513075764No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44430.2803572014508154No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC40420.25505374932797564No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC33740.21290236274928No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC32080.20242761698271794No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31460.1985153625397851No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT30630.19327798965650406No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC29380.18539037989252657No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA26240.16557670416541517No Hit
CCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCAC25410.16033933128213412No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA25150.1586987084512268No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24250.15301962942116304No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT22730.14342829594816642No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT22290.14065185731124633No Hit
GTGCTGCCCCTTTCACATCAAAGAACTACTGACAACGAAGGCCGCGCCTG22260.14046255467691088No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA21440.13528828267174167No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20690.13055571681335518No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCT19390.1223526026588186No Hit
GTCTTCTTCAGTTTCGACTTATCGAATTTCTCGATCTCAGCCATATCGGG18190.11478049728540023No Hit
GTGCTACACAGCTGTGGTCCCACTCGTATATGGTGGTGAGACCAAAATGG18020.11370778235749929No Hit
CTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGT17910.11301367269826927No Hit
CATTAGGATCTTCGGAAGAACGGATGATCCTGGAAGTAATCCGGGCACAC17710.11175165513603287No Hit
GTATCAACGCAGAGTACGGGAACTCGGTGGTGGCCACTGCGCAGACCAGA16580.10462125590939725No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCC16180.10209722078492445No Hit
GATTAAACTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTA15920.10045659795401712No Hit
GTCTATCTATCTGGCTGGCAGGGAAGGAAAGAACTTGCATGTTGGTGAAG15870.10014109356345803No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)