FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001963350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594794
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT131792.2157251081887175No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116391.9568119382508902No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113451.9073830603536686No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65321.0981953415804462No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41550.6985611825270598No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT30840.5184988416157527No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29650.4984919148478296No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20980.3527271626815334No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT14610.24563126057088672No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC14350.24125999926024808No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT14170.23823374142980594No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT12230.2056174070350407No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC12010.2019186474645003No Hit
CCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCAC11130.18712360918233875No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA11000.18493797852701943No Hit
GTGCTGCCCCTTTCACATCAAAGAACTACTGACAACGAAGGCCGCGCCTG10650.17905358830115972No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA10420.17518670329559477No Hit
CTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGT9820.16509917719412098No Hit
GTATCAACGCAGAGTACGGGAACTCGGTGGTGGCCACTGCGCAGACCAGA9640.16207291936367885No Hit
GATTAAACTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTA9130.15349852217742613No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8740.14694163021146817No Hit
CAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8620.1449241249911734No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCT8410.14139349085565758No Hit
GTCTATCTATCTGGCTGGCAGGGAAGGAAAGAACTTGCATGTTGGTGAAG7650.12861595779379079No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGACTTTTTTTTTTTT7590.1276072051836434Clontech Universal Primer Mix Long (95% over 23bp)
GTCTTCTTCAGTTTCGACTTATCGAATTTCTCGATCTCAGCCATATCGGG7410.12458094735320127No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC7340.12340406930802933No Hit
GAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGA7030.11819218082226787No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT6700.11264404146645729Illumina PCR Primer Index 10 (95% over 22bp)
TCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6630.11146716342128535No Hit
GAGTACGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA6600.11096278711621166No Hit
GGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGC6400.1076002784157204No Hit
GGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTC6160.10356526797513088No Hit
GCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTC6100.1025565153649835No Hit
TGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTG6000.10087526101473787No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)