FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001963355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4560497
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT276960.6073022304367265No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC216850.47549642067520276No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC200420.439469645523284No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC199020.43639980467041206No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA195400.42846207332227165No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC194630.4267736608531921No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG183590.4025657729848304No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT183390.40212722429156295No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG181430.3978294470975422No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT179030.39256686277833314No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG166090.36419276232393094No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA163720.35899596030871195No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT161450.3540184326401267No Hit
GCTTAAGAGTTACAGGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA159270.3492382518835118No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCG154360.3384718814637966No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC144310.3164348096271086No Hit
CTCTTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT143590.3148560343313459No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG140890.3089356269722357No Hit
GTAAGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAG136640.299616467240303No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT125100.27431220763877273No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAAGAGACAGAGTCACCATCACTT124760.2735666748602181No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG124400.27277728721233674No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG122970.26964166405547463No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC122940.2695758817514845No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG114800.25172694993550043No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC113110.24802121347739073No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC111820.24519257440581585No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT107890.236575092583111No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC107060.2347551155060512No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGT106340.2331763402102885No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGA103560.2270805133738713No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA103280.2264665452032969No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC103070.22600606907536613No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA101310.22214684057461279No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG101230.22197142109730583No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG98770.21657727217011657No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA98710.21644570756213632No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA98550.21609486860752236No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT94040.20620559557434198No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAAGT91770.20122806790575676No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT90620.19870641291946908No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT90010.19736883940500344No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA89270.19574620923991395No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC88380.19379466755487396No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG86220.18905834166758578No Hit
ACTTATTACTGTCAACAGCTTAAGAGTTACAGGCTCACTTTCGGCGGAGG84630.1855718795561098No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA84060.1843220157802976No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA81980.17976110937031642No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA81690.17912521376507867No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT81620.17897172172243508No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT81260.17818233407455372No Hit
ACTGTCAACAGCTTAAGAGTTACAGGCTCACTTTCGGCGGAGGGACCAAG81230.1781165517705636No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA80970.17754643846931595No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT80420.17634042956283053No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC79880.1751563480910085No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT79460.1742353958351469No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC77880.1707708611583343No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT77140.16914823099324483No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC77040.16892895664661112No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG76710.16820535130271985No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC76690.16816149643339312No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG75980.16660464857229376No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC75830.1662757370523432No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC75200.16489430866855082No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC74760.1639295015433625No Hit
AGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAA74740.16388564667403574No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA74170.16263578289822359No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT73590.16136399168774807No Hit
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCA73390.16092544299448064No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC72870.15978521639198534No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA72280.15849149774684645No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71700.15721970653637093No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGT70360.1542814302914792No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT70090.15368938955556818No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC69180.15169399300120143No Hit
GTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTG67540.14809789371640855No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATC66930.1467603202019429No Hit
GTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTG66720.14629984407401211No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG66190.14513769003685345No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCC65980.14467721390892266No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGT65100.142747599658546No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCCTGTAACTCTTAAGCTGTTGACAG64920.1423529058346053No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGC64280.14094955001614956No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT62220.1364324984754951No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCCTGTAACTCTTAAGCTGTTGACA61260.1343274647478115No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA61070.13391084348920743No Hit
ATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATT60870.13347229479594No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC60390.1324197779320982No Hit
CACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAAC60360.13235399562810807No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG59910.13136726106825639No Hit
GGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCC59750.13101642211364245No Hit
GAGTTACAGGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA59660.1308190752016721No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCA58390.12803429099942398No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGA58250.12772730691413678No Hit
GATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATG58240.12770537947947339No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT58210.1276395971754833No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57920.12700370157024554No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA57650.1264116608343345No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCC57470.12601696701039383No Hit
GTCTGCATCTGTAAGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG57340.12573191035977002No Hit
GTCTCTTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGT56780.1245039740186212No Hit
CCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCA56450.12378036867472997No Hit
GCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCCT56070.12294712615752187No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC55670.12207002877098702No Hit
CACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAGTTATTTAGCCTGGT55570.12185075442435331No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC54530.11957030121936271No Hit
GGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCC54450.11939488174205574No Hit
GCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTG54110.11864934896350111No Hit
GTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCA53960.11832043744355056No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA53720.11779417901162964No Hit
ATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATAC53090.11641275062783728No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC52920.11603998423855995No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAGTTATTTAGCC52770.11571107271860939No Hit
GTCAACAGCTTAAGAGTTACAGGCTCACTTTCGGCGGAGGGACCAAGGTG52700.1155575806759658No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG52410.11492168507072804No Hit
CTGTAAGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGC52300.11468048328943095No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC52130.11430771690015365No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC52080.1141980797268368No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT51820.11362796642558914No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG51750.11347447438294556No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC51610.11316749029765835No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA51570.11307978055900486No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC51430.11277279647371767No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT51240.11235617521511362No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA51130.11211497343381653No Hit
GTTACAGGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACT50920.11165449730588574No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG50720.11121594861261831No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT50640.11104052913531136No Hit
TTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGA50560.11086510965800439No Hit
CAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGT50400.11051427070339044No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG50050.10974681049017246No Hit
GCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCT49640.10884778566897424No Hit
GCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCTTACAGATGCAG48930.10729093780787488No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48450.10623842094403307No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCT48420.10617263864004298No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT48240.10577794481610228No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT48050.10536132355749823No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT47850.10492277486423081No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC47840.10490084742956743No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTC47340.10380447569639888No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCT47270.10365098365375529No Hit
ACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAA46970.10299316061385415No Hit
GCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAAGAGT46800.10262039422457685No Hit
GGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGCTTAGGGGCT46720.10244497474726988No Hit
GGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC46650.10229148270462626No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAG46620.10222570040063617No Hit
CCATCCTTCCTGTCTGCATCTGTAAGAGACAGAGTCACCATCACTTGCCG45650.10009873923828916No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)