FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001963361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6479691
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACAAACTTTTGGCCAGGGGACCA457890.7066540673004315No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT447650.6908508445850273No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT310830.47969880045205854No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC309840.4781709498184404No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC303040.4676766222339923No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC280670.4331533710480947No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC274470.42358501354462735No Hit
GTATGGTAGCTCACAAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC269850.41645504392107585No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT263360.40643913421180117No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG254300.3924569859889924No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT253620.3914075532305476No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA253390.3910525980328383No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC250010.38583629990998025No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT244770.3777494945360821No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT239710.369940480186478No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT237780.36696194309265673No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACAAACTTTTGGCCAGGGGAC236850.3655266894671366No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT236390.3648167790717181No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC229040.35347364557970434No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG217300.3353554976618484No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA211470.32635815504165244No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT208860.3223301851893864No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC204360.315385409582031No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG202910.31314764855299426No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG199580.30800851460355133No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA198380.3061565744415899No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT184870.2853068147848408No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCT183640.28340857611883036No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC180470.2785163675243156No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC178350.27524460657151706No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC172430.266108368439174No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA166650.2571881899923932No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG150930.23292777387069846No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG150800.2327271470198193No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA145750.22493356550489832No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA142940.22059693895897198No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA142680.22019568525721367No Hit
ACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGC139650.21551953634826107No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT139100.2146707304406954No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG138080.2130965813030282No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA137180.2117076261815571No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC136970.21138353665321385No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC136960.21136810381853088No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA136130.21008717853984085No Hit
GTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCT132550.2045622237233226No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT132090.20385231332790404No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG131070.20227816419023684No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA130190.20092007473813178No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG126300.19491670204644018No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC125250.19329625440472392No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT124960.1928487021989166No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT118250.18249327012661562No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC117460.18127407618665767No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA113650.17539416617243014No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC113390.17499291247067184No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC112230.17320270364744245No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC112110.1730175096312463No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC112070.17295577829251424No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA111400.17192177836875247No Hit
GTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGC109700.16929819647264044No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC109350.16875804725873503No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC108800.16790924135116936No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA108500.16744625631067903No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA108460.16738452497194697No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT107690.16619619670135505No Hit
GTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGAGCCTGG107300.16559431614871759No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT106100.16374237598675614No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA104700.16158177913113447No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG103410.15959094345702596No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC103230.15931315243273175No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA102680.15846434652516608No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC102440.1580939584927738No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT101670.15690563022218187No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC100510.1551154213989525No Hit
CCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCA100470.15505369006022046No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACC97940.15114918288541845No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA97200.15000715311887558No Hit
GGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGA96720.14926637705409102No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC96440.14883425768296665No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC96140.14837127264247632No Hit
CTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCA94110.1452384072018249No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT93230.14388031774971985No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG92360.14253766113229782No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCA92130.14218270593458854No Hit
TCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGC91580.1413339000270229No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC91080.14056225829287228No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCA90940.14034619860731012No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGG89930.13878748230432592No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC89050.1374293928522209No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGT87610.1352070646578672No Hit
GAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG87450.134960139302939No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG86780.1339261393791772No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC86240.13309276630629455No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTA84650.13063894559169564No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA83920.12951234865983577No Hit
ATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC82980.12806166219963266No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGT82450.12724372196143305No Hit
CAGTATGGTAGCTCACAAACTTTTGGCCAGGGGACCAAGCTGGAGATCAA81730.12613255786425617No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT81200.12531461762605656No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA80940.12491336392429825No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGAC80870.12480533408151714No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTG80560.1243269162063438No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA79720.12303055809297078No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGT78770.12156443879808465No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT78690.12144097612062058No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC78320.12086996123734912No Hit
GGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAA78280.12080822989861707No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC77710.11992855832168539No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCA77390.11943470761182902No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG77330.11934211060373094No Hit
CACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGC76880.11864763304299542No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC76870.11863220020831242No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA76200.1175982002845506No Hit
GCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGT74970.11569996161854014No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74800.11543760342892895No Hit
GGCCAAAAGTTTGTGAGCTACCATACTGCTGACAGTAATACACTGCAAAA74140.11441903633985016No Hit
GGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGACTGGC73890.11403321547277485No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT73770.11384802145657871No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTTTGTGA73520.1134622005895034No Hit
ATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTG73420.1133078722426733No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG73360.11321527523457522No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT73080.1127831558634509No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT72890.11248993200447367No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG72580.1120115141293003No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC72560.11198064845993427No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA72190.11140963357666285No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG71040.10963485758811646No Hit
CTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAG70730.1091564397129431No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT70620.10898667853142999No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA70580.10892494719269794No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG70030.10807614128513228No Hit
ATGGTAGCTCACAAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA69800.107721186087423No Hit
GGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC69710.1075822905752759No Hit
CTCCAGCTTGGTCCCCTGGCCAAAAGTTTGTGAGCTACCATACTGCTGAC69690.10755142490590987No Hit
ACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC68160.10519020119940904No Hit
CTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGC68070.10505130568726194No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCC67590.10431052962247737No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT67430.10406360426754918No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG66530.10267464914607811No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAG66050.10193387308129354No Hit
GGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA65860.10164064922231632No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTG65370.10088444032284874No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)