FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001963399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967464
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80610.8332092977103025No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70820.7320169019208984No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT70150.7250915796350045No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37070.38316671214639514No Hit
GTGCTGCCCCTTTCACATCAAAGAACTACTGACAACGAAGGCCGCGCCTG30970.3201152704390034No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC28820.29789222131262766No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT26160.27039765820743716No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23950.24755443096590676No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC23920.24724434190832942No Hit
CCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCAC22480.2323600671446173No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT21330.22047331993748603No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA20740.21437490180513177No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA19960.20631258630812102No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18850.19483929117775958No Hit
CTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGT17710.18305590698982083No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16620.17178933789784426No Hit
GATTAAACTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTA15090.1559747959614001No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCT14750.15246045330885696No Hit
GTCTATCTATCTGGCTGGCAGGGAAGGAAAGAACTTGCATGTTGGTGAAG14640.15132346009774006No Hit
GTCTTCTTCAGTTTCGACTTATCGAATTTCTCGATCTCAGCCATATCGGG14620.15111673405935516No Hit
GAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGA12760.13189121248956034No Hit
GGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTC12000.12403562303093449No Hit
GTATCAACGCAGAGTACGGGAACTCGGTGGTGGCCACTGCGCAGACCAGA11930.12331208189658738No Hit
GCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTC11190.11566321847634639No Hit
TGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTG10970.11338923205411261No Hit
GGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGC10630.10987488940156946No Hit
GCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTG10440.10791099203691301No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10200.10543027957629432No Hit
GTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACTGACAACGA9920.10253611503890585No Hit
CTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCG9750.10077894371263428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)