FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001963400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503578
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT130732.5960228604109No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130242.58629249093487No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119332.369642835866539No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65591.3024794570056675No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT57361.139048965602151No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42240.8387975646275254No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31730.6300910683151368No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22760.45196573321312683No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT14820.29429403190766873No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT12910.2563654488480434No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT12740.2529896063767678TruSeq Adapter, Index 11 (95% over 23bp)
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11890.2361103940203901No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11160.22161412929079505No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT11090.22022407650850515RNA PCR Primer, Index 11 (96% over 25bp)
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC9650.19162870498711224No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9610.19083438911151798No Hit
CAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9080.1803097037598942No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8990.17852249303980716No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC8940.17752959819531433No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT8880.17633812438192295No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8570.17018217634606753No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8240.163629070372415No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8050.1598560699633423No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7540.14972854254951565No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7480.1485370687361243No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7180.14257969966916742No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT7070.14039533101128326No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGACTTTTTTTTTTTT7070.14039533101128326Clontech Universal Primer Mix Long (95% over 23bp)
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6940.13781380441560195No Hit
GTATCAACGCAGAGTACGGGAGAAGAAGTGAAGTCAAGATGAAGAACCAT6930.13761522544670338No Hit
TCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6700.13304790916203646No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA6700.13304790916203646No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA6550.13006922462855805No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG6460.12828201390847097No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC6460.12828201390847097No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6450.12808343493957242No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA6110.12133174999702133No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCC5990.11894880237023858No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC5900.11716159165015151No Hit
CATTAGGATCTTCGGAAGAACGGATGATCCTGGAAGTAATCCGGGCACAC5800.1151758019611659No Hit
CTCGTATATGGTGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGA5390.10703406423632486No Hit
GTGCTACACAGCTGTGGTCCCACTCGTATATGGTGGTGAGACCAAAATGG5310.10544543248513637No Hit
GTCTTCAGGCTGCAGGCCGCTGATGGTGAGAGCGAATTCTGTCCCAGATC5300.10524685351623779No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5290.10504827454733924No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC5240.10405537970284644No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA5220.1036582217650493No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)