FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001963402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26200
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT16096.141221374045801No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8363.1908396946564888No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8303.16793893129771No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8153.1106870229007635No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4651.7748091603053435No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT4571.7442748091603053TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT4431.6908396946564885RNA PCR Primer, Index 11 (95% over 23bp)
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3391.2938931297709924No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.6603053435114503No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.6412213740458015No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT1350.5152671755725191TruSeq Adapter, Index 11 (96% over 26bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG1170.44656488549618323TruSeq Adapter, Index 11 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1050.40076335877862596No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.29770992366412213No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT760.2900763358778626No Hit
CAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.2595419847328244No Hit
TCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.2519083969465649No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG650.2480916030534351No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT640.24427480916030536No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG640.24427480916030536TruSeq Adapter, Index 11 (95% over 24bp)
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA630.24045801526717558No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT500.19083969465648853No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC500.19083969465648853No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGT480.183206106870229No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA450.1717557251908397RNA PCR Primer, Index 11 (96% over 28bp)
CGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.16412213740458015No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC430.16412213740458015No Hit
AACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.15648854961832062No Hit
GGTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCA390.14885496183206107No Hit
GTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.1450381679389313No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAAC370.14122137404580154No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGT370.14122137404580154No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA360.13740458015267176No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.13740458015267176No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT360.13740458015267176No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.1297709923664122No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGT300.11450381679389314No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)