FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001963403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26200
Sequences flagged as poor quality0
Sequence length151
%GC36

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4551.7366412213740456No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4371.6679389312977098No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3921.4961832061068703No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.8206106870229009No Hit
CTTATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTG1840.7022900763358778No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.5114503816793893No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.3893129770992366No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.33969465648854963No Hit
ATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTC840.32061068702290074No Hit
CATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTCGCCGT780.29770992366412213Illumina RNA PCR Primer (96% over 25bp)
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.2480916030534351No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT570.21755725190839695No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA550.20992366412213742No Hit
CGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.18702290076335878No Hit
CAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.16030534351145037No Hit
TCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.15267175572519084No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAAC390.14885496183206107No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC380.1450381679389313No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT330.12595419847328246No Hit
GGTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCA330.12595419847328246No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.12213740458015268No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.12213740458015268No Hit
GTATCAACGCAGAGTACGGGCGACTGCGTGAAGTCGGAATCGCTAGTAAT280.1068702290076336No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT270.10305343511450382No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)