FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001963418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359506
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT121583.3818628896318836No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84472.3496130801711237No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72172.007476926671599No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT71251.9818862550277323No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39861.1087436649179707No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT31650.880374736443898RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT28660.7972050536013308RNA PCR Primer, Index 29 (96% over 25bp)
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26760.7443547534672579No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20390.5671671682809188No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18390.5115352734029474No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT9120.25368144064354975RNA PCR Primer, Index 29 (96% over 28bp)
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8900.2475619322069729No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8080.22475285530700462No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG8070.22447469583261478RNA PCR Primer, Index 29 (96% over 30bp)
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT7140.198605864714358No Hit
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC6600.18358525309730575No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6180.17190255517293174No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6030.16773016305708388No Hit
CAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.1579945814534389No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5670.15771642197904903No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5510.15326587038881131No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTG5390.14992795669613304Illumina PCR Primer Index 10 (96% over 25bp)
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5080.14130501299004747No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4970.13824525877175903No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC4740.1318475908607923No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.12962231506567345No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCT4620.12850967716811404RNA PCR Primer, Index 29 (95% over 22bp)
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG4500.12517176347543574No Hit
TCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.1215556903083676No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.11877409556446902No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG4110.1143235439742313No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT4090.11376722502545158No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC4060.11293274660228202No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT3750.10430980289619644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)