FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001963421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110250
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGACTTAATAGGTGTAGATCTCGGTG13781.2498866213151927No Hit
ATACACATCTGACGCTGCCGACGACTTAATAGGTGTAGATCTCGGTGGTC6720.6095238095238096No Hit
CATCTGACGCTGCCGACGACTTAATAGGTGTAGATCTCGGTGGTCGCCGT2440.22131519274376418Illumina Single End PCR Primer 1 (100% over 23bp)
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT1700.15419501133786848No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC1320.11972789115646258No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC1280.11609977324263038No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT1250.11337868480725624No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.11337868480725624No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC1200.10884353741496598No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC1180.10702947845804989No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.10521541950113378No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT1140.1034013605442177No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.1034013605442177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)