FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001963456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535496
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90211.684606420962995No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85681.6000119515365194No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT75911.4175642768573435No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39700.7413687497198859No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28550.533150574420724No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT24920.4653629532246739No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19800.3697506610693637No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17750.3314683956556165No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC8550.15966505818904342No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7880.14715329339528213No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC7720.1441654092654287No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT7290.13613547066644754No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA7230.13501501411775252No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6910.1290392458580456No Hit
CCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCAC6890.12866576034181396No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA6540.12212976380775954No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT6350.11858165140355857No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGACTTTTTTTTTTTT6310.1178346803710952Clontech Universal Primer Mix Long (95% over 23bp)
CAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5950.11111194107892497No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCT5680.10606988660979727No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT5540.10345548799617552No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5380.10046760386632206No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)