FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001963459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15417
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGACTTAATAGGTGTAGATCTCGGTG4102.6594019588765647No Hit
ATACACATCTGACGCTGCCGACGACTTAATAGGTGTAGATCTCGGTGGTC1601.0378153985859764No Hit
CATCTGACGCTGCCGACGACTTAATAGGTGTAGATCTCGGTGGTCGCCGT820.531880391775313Illumina Single End PCR Primer 1 (100% over 23bp)
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.5124213530518259No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.5124213530518259No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.4864759680871765No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.21404942595835766No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.20107673347603294No Hit
TATACACATCTGACGCTGCCGACGACTTAATAGGTGTAGATCTCGGTGGT230.1491859635467341No Hit
CACATCTGACGCTGCCGACGACTTAATAGGTGTAGATCTCGGTGGTCGCC200.12972692482324705Illumina Single End PCR Primer 1 (100% over 21bp)
TTATACACATCTGACGCTGCCGACGACTTAATAGGTGTAGATCTCGGTGG200.12972692482324705No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.12324057858208472No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT160.10378153985859766No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.10378153985859766No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)