FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001963464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238072
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT327781.4645641427085456No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279331.2480831715869731No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238101.0638621098874388No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171660.7669994531007045No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT118460.52929485735937No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88680.3962339013222095No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77750.3473972240392624No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC45730.2043276534445719No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT44380.19829567592106065No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT42720.19087857763289115No Hit
CCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCAC40350.18028910598050465No Hit
GTATCAACGCAGAGTACGGGAACTCGGTGGTGGCCACTGCGCAGACCAGA40210.17966356757065902No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40120.17926143573575828No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT35190.15723354744619475No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA34950.1561611958864594No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC33840.15120156992268344No Hit
GATTAAACTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTA33160.14816324050343332No Hit
CTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGT31150.1391822961906498No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA31010.1385567577808042No Hit
GTGCTGCCCCTTTCACATCAAAGAACTACTGACAACGAAGGCCGCGCCTG30370.13569715362150994No Hit
GAGTACGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA26840.11992464943040261No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT26510.1184501660357665TruSeq Adapter, Index 7 (95% over 21bp)
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCT25350.11326713349704567No Hit
GGTATCAACGCAGAGTACGGGAACTCGGTGGTGGCCACTGCGCAGACCAG23140.10339256288448272No Hit
GAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGA22390.10004146426030977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)