FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001963466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581447
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT63821.0976064886395491No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40700.6999778139710068No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39410.6777917849778226No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36190.6224127048553006No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT25730.44251668681754314No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC24000.41276333010575345No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22610.3888574539037952No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC21520.37011111932815893No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA20990.36099592912165684No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT17750.3052728795573801No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17690.30424097123211574No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA17410.2994253990475486No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT17350.2983934907222842Illumina Paired End PCR Primer 2 (95% over 22bp)
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16990.29220204077069795No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC16950.29151410188718835No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT16530.2842907436103376Illumina Paired End PCR Primer 2 (95% over 24bp)
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG15310.26330860766329517No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14970.2574611271534637No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA14770.25402143273591576No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT14530.24989379943485818No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG14370.24714204390081984No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14070.24198250227449794No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13880.2387147925778274No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13790.23716693008993078No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGT13640.23458715927676985No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC13180.22667586211640955No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC13010.22375212186149385No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC12240.21050929835393423No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12240.21050929835393423No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11650.20036219982216782No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11650.20036219982216782No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG11630.200018230380413No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG11500.19778242900900683No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG11480.19743845956725203No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCA11160.19193494849917533No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11130.19141899433654316No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT10990.18901120824425957No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10970.1886672388025048No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC10770.18522754438495684No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG10760.18505555966407947No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10660.18333571245530547No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGCACTCAGG10620.18264777357179587No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA10570.1817878499674089No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10550.1814438805256541No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGC10540.1812718958047767No Hit
GCATGGGATGACAGCCTGAGTGCTTATGTCTTCGGAACTGGGACCAAGGT10340.17783220138722877No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC10290.17697227778284177No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTC10210.17559640001582258No Hit
GTGCAGCATGGGATGACAGCCTGAGTGCTTATGTCTTCGGAACTGGGACC9930.17078082783125548No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCC9480.16304151539177258No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9450.16252556122914041No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC9360.16097769874124382No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC9290.15977380569510205No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATT9280.15960182097422465No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9270.15942983625334725No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9050.15564617239404452No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT9010.15495823351053492No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT8990.15461426406878012No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8950.15392632518527055No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTG8940.15375434046439312No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG8910.15323838630176095No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT8850.15220647797649658No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGCTTATGTCTTCGG8830.15186250853474179No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG8520.14653098218754246No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGCACTCAGGC8510.1463589974666651No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC8400.1444671655370137No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT8190.14085548639858836No Hit
AGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAG8130.13982357807332396No Hit
GTGCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC7960.1368998378184082No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG7950.13672785309753083No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG7940.13655586837665343No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC7840.13483602116787946No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG7840.13483602116787946No Hit
CTCTAACCCCACCCACGGGAGCCTGGAGCTGCAGGATCCCAGGGGAGGGG7830.13466403644700203No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA7760.13346014340086026No Hit
AGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGA7710.1326002197964733No Hit
CTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGC7680.1320842656338411No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA7650.1315683114712089No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGTGG7570.13019243370418973No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCC7470.12847258649541576No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGA7370.1267527392866418No Hit
TGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGA7350.12640876984488697No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA7330.12606480040313217No Hit
CTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACC7190.12365701431084862No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA7110.12228113654382945No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7100.12210915182295207No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG7090.12193716710207465No Hit
GGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG6990.12021731989330067No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC6980.12004533517242329No Hit
GCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTAC6970.11987335045154589No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC6900.11866945740540412No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA6860.11798151852189451No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC6860.11798151852189451No Hit
GAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTT6840.11763754908013972No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG6770.11643365603399794No Hit
CAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGA6610.11368190049995959No Hit
CACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCC6570.11299396161645No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC6530.11230602273294042No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAGGAATAATCAGCGGCCCT6440.11075816024504384No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTG6330.10886632831539247No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGG6300.10835037415276028No Hit
GTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGCCTGGAGCTGCAG6290.10817838943188286No Hit
GTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAG6290.10817838943188286No Hit
GGATGACAGCCTGAGTGCTTATGTCTTCGGAACTGGGACCAAGGTCACCG6280.10800640471100548No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC6270.10783441999012808No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG6240.10731846582749589TruSeq Adapter, Index 5 (96% over 28bp)
GTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGA6240.10731846582749589No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCC6160.1059425880604767No Hit
CTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGA6150.10577060333959931No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG6150.10577060333959931No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA6120.10525464917696711No Hit
GCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAA6110.10508266445608971No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCT6110.10508266445608971Illumina Paired End PCR Primer 2 (96% over 27bp)
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG6080.10456671029345753No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTC6030.10370678668907055No Hit
GTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAA5990.10301884780556095No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTC5980.10284686308468355No Hit
GGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCC5980.10284686308468355No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTT5960.10250289364292878No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCC5960.10250289364292878No Hit
TACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT5910.10164297003854178No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGAT5880.10112701587590958No Hit
ACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGC5880.10112701587590958No Hit
TGGGATGACAGCCTGAGTGCTTATGTCTTCGGAACTGGGACCAAGGTCAC5820.1000951075506452No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)