FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001963467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581447
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT22490.38679363725326643No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC20540.35325661668217395No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA19230.33072661824723487No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18090.3111203600672116No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17960.3088845586958055No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17490.3008012768145678No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT16030.2756915075664678No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15870.27293975203242943No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC15670.2695000576148815No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15530.2670922715225979No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15340.26382456182592734No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14730.2533334938524061No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG14600.25109769248099995No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA12790.21996845800219106No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12660.21773265663078495No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGT12530.21549685525937878No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT12360.212573115004463No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC11820.20328594007708356No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11510.1979544137298842No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC11470.19726647484637466No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC11420.19640655124198766No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11240.1933108262661945No Hit
CTTATACACATCTGACGCTGCCGACGACTTAATAGGTGTAGATCTCGGTG11050.19004311656952397No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11010.18935517768601437No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC10910.1876353304772404No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10880.1871193763146082No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCA10720.18436762078056984No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10700.18402365133881507No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10550.1814438805256541No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10380.17852014027073834No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG10220.17576838473669998No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10100.17370456808617124No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT9940.17095281255213288No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9810.1687170111807267No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG9710.16699716397195272No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC9680.16648120980932052No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGCTTATGTCTTCGG9590.16493334732142398No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTC9580.16476136260054655No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9500.16338548483352738No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9260.15925785153246985No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATT9170.15770998904457328No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGC9110.1566780807193089No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC9050.15564617239404452No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCC9030.15530220295228972No Hit
GTGCAGCATGGGATGACAGCCTGAGTGCTTATGTCTTCGGAACTGGGACC8960.15409830990614795No Hit
GCATGGGATGACAGCCTGAGTGCTTATGTCTTCGGAACTGGGACCAAGGT8930.15358235574351575No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8920.15341037102263835No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGCACTCAGG8830.15186250853474179No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8780.1510025849303548No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC8720.14997067660509042No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8700.1496267071633356No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTG8650.14876678355894862No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT8540.14687495162929726No Hit
GGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG8330.1432632724908719No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC8010.1377597614227952No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT7930.13638388365577603No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG7900.13586792949314383No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG7900.13586792949314383No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGCACTCAGGC7830.13466403644700203No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG7780.13380411284261506No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7750.13328815867998287No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT7710.1326002197964733No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG7690.1322562503547185No Hit
CTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGC7650.1315683114712089No Hit
TGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGA7600.1307083878668219No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGTGG7560.13002044898331233No Hit
GTGCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC7470.12847258649541576No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG7460.12830060177453834No Hit
AGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAG7300.12554884624049997No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC7230.1243449531943582No Hit
AGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGA7170.12331304486909384No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTC7150.12296907542733904No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT7150.12296907542733904No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC7060.12142121293944245No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC7030.12090525877681026No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7000.12038930461417809No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC6990.12021731989330067No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAGGAATAATCAGCGGCCCT6930.11918541156803629No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG6880.1183254879636493No Hit
GCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTAC6770.11643365603399794No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA6600.11350991577908219No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCC6590.11333793105820478No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA6520.11213403801206301No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA6480.11144609912855342No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG6390.10989823664065684No Hit
GGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGGAAT6360.10938228247802464No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6320.10869434359451506No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGA6320.10869434359451506No Hit
CTCTAACCCCACCCACGGGAGCCTGGAGCTGCAGGATCCCAGGGGAGGGG6310.10852235887363766No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG6280.10800640471100548No Hit
GGATGACAGCCTGAGTGCTTATGTCTTCGGAACTGGGACCAAGGTCACCG6240.10731846582749589No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC6210.1068025116648637No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA6200.10663052694398631No Hit
CTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACC6070.10439472557258013No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCCCAACTCTAACC6010.10336281724731575No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTG5940.10215892420117396No Hit
TACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT5930.10198693948029658No Hit
GTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGCCTGGAGCTGCAG5880.10112701587590958No Hit
CACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCC5850.10061106171327738No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA5850.10061106171327738No Hit
TTACTGTGCAGCATGGGATGACAGCCTGAGTGCTTATGTCTTCGGAACTG5840.1004390769924No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG5840.1004390769924No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)