FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001963469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4223167
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT249890.5917123334218135No Hit
GATCTATACTGCATCCAGTTTGCAAACTGGGGTCCCATCCAGGTTCAGCG241590.5720588364135257No Hit
GGCTAACAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA237200.5616637940199855No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC220120.5212202122246172No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC210930.4994592920431515No Hit
GTATAGATCAGGAGTTTAGGGGCTTTCCCTGCTTTCTGCTGATACCAGGC209510.49609688653089024No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA204270.48368913661240487No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC193830.45896835242366685No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC184110.43595244990311777No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT182650.43249532874262375No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC181500.4297722538559332No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG165750.3924779673643026No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA165370.39157816870609186No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG161700.3828880079807405No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGACTGCAGGCT160190.3793124922599556No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT153650.36382648377390714No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG150420.3561781951791156No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAAGCAGGGAAAGCCC144360.3418287744718596No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT144030.34104737037393973No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC142200.3367141294672932No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG140730.333233329394741No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC131210.31069100511535536No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC127140.3010536879076769No Hit
ATACTGCATCCAGTTTGCAAACTGGGGTCCCATCCAGGTTCAGCGGCAGT122430.28990092032827497No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT118980.2817316956682035No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAG115930.27450962749045915No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG113660.26913451445325276No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC112880.26728755931271486No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA103260.24450844591274748No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC100350.23761788250381763No Hit
ATTGTCAACAGGCTAACAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAG98810.23397133004685822No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA98290.23274002661983292No Hit
CACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAACTTACTATTGTCAAC96930.2295196945799207No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT95020.2249970223768087No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC94610.22402618698242338No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG94360.22343421418096893No Hit
GTCCCAGATCCACTGCCGCTGAACCTGGATGGGACCCCAGTTTGCAAACT89950.21299181396331238No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA88170.20877696761695666No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTGGATGGGACCCCA88050.2084928206722585No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC87360.20685897574024426No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA87180.20643275532319702No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAACTTA86980.20595917708203346No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG86390.20456212127060094No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC85910.20342553349180842No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC84120.1991870082333945No Hit
GCTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAG83390.1974584476531475No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT82190.19461697820616614No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG82180.19459329929410796No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT81870.19385925302030443No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT81800.1936935006358972No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA81700.1934567115153154No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA81530.19305417001032638No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT80790.1913019305180212No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC80320.19018902165128682No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC80190.1898811957945305No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA79990.18940761755336694No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC78170.1850980555587785No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA76980.18228026502385533No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC75840.17958086904922302No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA75650.1791309697201176No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG74520.1764552526575435No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC74380.176123747888729No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC71800.17001458857771903No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTGG71760.16991987292948632No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG71650.16965940489684636No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT71400.1690674320953919No Hit
GTCTCCTACAGATGCAGACACGGAAGATGGAGACTGGGTCATCTGGATGT71140.1684517803818793No Hit
GTTTAGGGGCTTTCCCTGCTTTCTGCTGATACCAGGCTAACCAGCTGCTA71020.16816763343718116No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTG69660.1649473013972689No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGT68260.16163225370912399No Hit
CCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG68250.1616085747970658No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGGAAACTGTTAGCCTGTTGACAA67870.16070877613885504No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC67750.1604246291941569No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT67100.15888549991037532No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC66910.15843560058126993No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC66860.15831720602097904No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT66730.15800938016422272No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG66640.15779626995569912No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG66300.15699118694572106No Hit
ATGCAGTATAGATCAGGAGTTTAGGGGCTTTCCCTGCTTTCTGCTGATAC65550.1552152685413577No Hit
CTCCTGATCTATACTGCATCCAGTTTGCAAACTGGGGTCCCATCCAGGTT63600.15059788069001295No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA63300.14988751332826764No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT62520.14804055818772974No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61800.1463356765195409No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG61680.14605152957484277No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT61190.14489126288399204No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT61040.14453607920311937No Hit
CTGGGGTCCCATCCAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT61000.14444136355488665No Hit
GTCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTCCCGT59570.14105527913056717No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGT58840.13932671855032017No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGG58760.13913728725385477No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58220.13785862600271315No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC57360.1358222395657098No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA57360.1358222395657098No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG57200.13544337697277897No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCA56710.13428311028192821No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC56430.13362010074429923No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC56160.13298077011872844No Hit
GTCCCATCCAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCAC56030.13267294426197213No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA55860.13227040275698307No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT55490.1313942830108305No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC55350.131062778242016No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGCTTTCTGCTGATACCAGGCTAACC55340.13103909932995783No Hit
GGATGGGACCCCAGTTTGCAAACTGGATGCAGTATAGATCAGGAGTTTAG55290.13092070476966694No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC54610.12931053874971082No Hit
GGTTAGCCTGGTATCAGCAGAAAGCAGGGAAAGCCCCTAAACTCCTGATC54360.12871856594825637No Hit
ACTTACTATTGTCAACAGGCTAACAGTTTCCCGTGGACGTTCGGCCAAGG53600.12691896863183483No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT53170.12590077541333317No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT52210.12362759985574806No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT52140.12346184747134081No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC51280.12142546103433749No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG50680.1200047263108468No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT50390.11931803786115966No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA50280.1190575698285197No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCC50200.11886813853205426No Hit
TTACTATTGTCAACAGGCTAACAGTTTCCCGTGGACGTTCGGCCAAGGGA50040.11848927593912341No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT49660.11758947728091264No Hit
CCCCTAAACTCCTGATCTATACTGCATCCAGTTTGCAAACTGGGGTCCCA49220.11654760515035281No Hit
AGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAAGCAGGGAA49100.11626345820565467No Hit
TCTATACTGCATCCAGTTTGCAAACTGGGGTCCCATCCAGGTTCAGCGGC48930.11586091670066564No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC48730.11538733845950207No Hit
GGTATCAGCAGAAAGCAGGGAAAGCCCCTAAACTCCTGATCTATACTGCA48710.11533998063538572No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48430.11467697109775674No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT48280.11432178741688406No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCT48200.11413235612041862No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC48100.11389556699983686No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA47820.11323255746220787No Hit
CAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA47810.11320887855014967No Hit
GTCCACGGGAAACTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTC47790.11316152072603333No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC47760.1130904839898588No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAG47750.11306680507780062No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG47500.11247483227634616No Hit
ATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATC47040.11138560232166997No Hit
CTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGC47000.11129088667343726No Hit
CAATAGTAAGTTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGT46550.11022533563081925No Hit
GTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAAGCAGGGAAAGCCCCT46400.10987015194994657No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG46400.10987015194994657No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC46130.10923082132437577No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG45710.10823630701793227No Hit
ATAGTAAGTTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGA45110.10681557229444158No Hit
GGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTA44820.10612888384475443No Hit
GCCTGGTATCAGCAGAAAGCAGGGAAAGCCCCTAAACTCCTGATCTATAC44620.10565530560359085No Hit
GCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAA44600.1056079477794745No Hit
GCTGATACCAGGCTAACCAGCTGCTAATACCCTGACTCGCCCGACAAGTG44420.1051817273624273No Hit
CCATCAGCAGCCTGCAGTCTGAAGATTTTGCAACTTACTATTGTCAACAG44240.10475550694538009No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT43970.1041161763198093No Hit
CTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGC43850.10383202937511114No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT43690.10345316678218028No Hit
GTCCCTTGGCCGAACGTCCACGGGAAACTGTTAGCCTGTTGACAATAGTA43590.10321637766159851No Hit
CTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCCAGATGACCCAGTC43570.10316901983748215No Hit
CTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAACTTACTATTGTCA43570.10316901983748215No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC43190.10226922117927137No Hit
GCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGTCT43170.10222186335515503No Hit
GCTTTCTGCTGATACCAGGCTAACCAGCTGCTAATACCCTGACTCGCCCG42890.10155885381752604No Hit
GGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACAC42640.10096688101607158No Hit
ACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAA42420.10044594495079166No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)