FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001963472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9005409
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT463370.5145463132213096No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC413830.4595349306178098No Hit
GTCCTAAACTCCTCATCCGTGCTGCATCCATTTTGGAAACAGGGGTCCCA385340.42789838862399254No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA367730.40834347446073793No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365650.4060337514931304No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC339180.3766403058428551No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC337600.37488580474246086No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC336690.37387530094413257No Hit
CTATTACAGTGCCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA315520.35036720708631885No Hit
GTTTAGGACCTGTCCCCGATTTCTCCTGATACCAGGCTATATAATTGGCA303870.3374305375802476No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT282140.313300595231155No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG277770.30844795611171016No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT266060.2954446599815733No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG265960.29533361560813065No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT261900.29082521404635814No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA257660.28611693261238885No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT253840.28187503754687876No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG250810.2785103930315658No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG246240.2734356651652357No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC245180.27225859480674336No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227930.2531034403878824No Hit
CTGTAATAGTTTTGACAGTGATAAGTTGCAACATCCTCAGGTTGCAGGCT222970.24759563946512592No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG222730.24732913296886347No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT212200.2356361604453501No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC211170.23449240339889058No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT200490.22263286431521323No Hit
CACCATCACCAGCCTGCAACCTGAGGATGTTGCAACTTATCACTGTCAAA194210.2156592776630134No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC185290.2057541195519271No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA184400.2047658246282873No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG175140.19448311564749585No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171920.19090748682264183No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT170350.18916409015959185No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC165750.18405604898122896No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTGCCAA165390.18365628923683533No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA160010.1776821019456196No Hit
GGTCCTAAACTCCTCATCCGTGCTGCATCCATTTTGGAAACAGGGGTCCC159510.1771268800784062No Hit
CTCCTCATCCGTGCTGCATCCATTTTGGAAACAGGGGTCCCATCTCGATT159310.17690479133152087No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT159230.17681595583276674No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC158960.17651613602447153No Hit
AGTCAGGGCATTGCCAATTATATAGCCTGGTATCAGGAGAAATCGGGGAC158920.17647171827509445No Hit
GTGATAAGTTGCAACATCCTCAGGTTGCAGGCTGGTGATGGTGAGAGTGA156240.17349572906683083No Hit
GCCCTGACTCGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAG155860.17307376044774866No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT155720.17291829832492894No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT154940.1720521522120761No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG154030.1710416484137478No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA147960.16430125494577758No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA147680.1639903307001381No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC146480.16265779821882603No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA146330.16249123165866203No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT146110.16224693403708815No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC146020.16214699410098976No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC143850.15973733119728376No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC143160.15897112502052932No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG141420.15703895292262685No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA139890.15533997400895397No Hit
GATGAGGAGTTTAGGACCTGTCCCCGATTTCTCCTGATACCAGGCTATAT138170.15343001078573998No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC135270.15020972395590249No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT135080.14999873964636143No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG134840.149732233150099No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG134620.14948793552852513No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG133440.14817761192190163No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC133090.14778895661485225No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGG131310.1458123667675727No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG130750.14519051827629373No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT127070.14110408533360339No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCC126580.14055996790373432No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC125150.13897203336350408No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT124820.13860558693114328No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA124740.13851675143238912No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC123950.13763950088219204No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG123140.13674004145730637No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCT120810.13415270755609213No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG119570.13277575732540298No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAATCGAGA119340.13252035526648484No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT118110.13115450947313997No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAATCGAGATGGGACCCCT117780.13078806304077914No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC116960.12987749917854924No Hit
GATTTCACTCTCACCATCACCAGCCTGCAACCTGAGGATGTTGCAACTTA116040.12885589094287667No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC116000.12881147319349961No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT115970.1287781598814668No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA115900.12870042882005694No Hit
GTCCCAGATCCACTGCCACTGAATCGAGATGGGACCCCTGTTTCCAAAAT114710.12737900077608913No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA114410.12704586765576112No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGT113850.12642401916448215No Hit
ACAGTGCCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA109900.12203776641349659No Hit
TCCGTGCTGCATCCATTTTGGAAACAGGGGTCCCATCTCGATTCAGTGGC109820.12194893091474247No Hit
ATCGGGGACAGGTCCTAAACTCCTCATCCGTGCTGCATCCATTTTGGAAA108190.12013890762762691No Hit
GGTTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC102900.11426466027250955No Hit
GTCTCCGACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT102110.11338740972231245No Hit
GCCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGT101610.11283218785509909No Hit
GCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACATCC100690.11181057961942652No Hit
GTAATAGTTTTGACAGTGATAAGTTGCAACATCCTCAGGTTGCAGGCTGG100460.11155517756050837No Hit
GGTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAATCGAG100290.11136640212565582No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100210.11127756662690168No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC100150.11121094000283607No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA99320.11028927170326189No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA99240.11020043620450774No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT98010.1088345904111629No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA96990.10770193780204762No Hit
GTATCAGGAGAAATCGGGGACAGGTCCTAAACTCCTCATCCGTGCTGCAT95110.10561430358132541No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT92250.10243843450086498No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT91550.10166112388676628No Hit
ATATAATTGGCAATGCCCTGACTCGCCCGGCAAGTGATGGTGACTCTGTC91350.10143903513988094No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG90240.10020644259466727No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC90200.1001620248452902No Hit
GTGCCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACT90200.1001620248452902No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)