FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001963473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9005409
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT388640.4315628529476008No Hit
GTCCTAAACTCCTCATCCGTGCTGCATCCATTTTGGAAACAGGGGTCCCA347070.38540170690748193No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC337740.3750412668652806No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA335040.37204306878232846No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC308990.3431160095005124No Hit
CTATTACAGTGCCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA287790.3195746023306659No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT280610.3116016163174821No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC275790.30624927751754527No Hit
GTTTAGGACCTGTCCCCGATTTCTCCTGATACCAGGCTATATAATTGGCA269030.29874267787282066No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC247630.27497918195608884No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA238280.264596533039199No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG236230.26232012338362426No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG234310.2601880714135249No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG228280.2534920956949318No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT226330.2513267304127997No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT221070.24548579636971515No Hit
CTGTAATAGTTTTGACAGTGATAAGTTGCAACATCCTCAGGTTGCAGGCT220980.24538585643361674No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC218830.24299840240459927No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG208650.23169408518813528No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG195150.21670309477337454No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC191150.21226131983566762No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC185890.20642038579258312No Hit
CACCATCACCAGCCTGCAACCTGAGGATGTTGCAACTTATCACTGTCAAA185070.2055098219303532No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT183450.2037109030805819No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT176900.1964374966200869No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC172630.19169590187408478No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG160600.17833726374893133No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA154650.17173012352909237No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTGCCAA151840.16860977663535326No Hit
AGTCAGGGCATTGCCAATTATATAGCCTGGTATCAGGAGAAATCGGGGAC149170.16564489186443393No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA148910.16535617649348297No Hit
GGTCCTAAACTCCTCATCCGTGCTGCATCCATTTTGGAAACAGGGGTCCC146680.16287988696571137No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC145780.16188048760472734No Hit
CTCCTCATCCGTGCTGCATCCATTTTGGAAACAGGGGTCCCATCTCGATT144200.1601259865043331No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143250.15907106495662773No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC141200.15679465530105294No Hit
GCCCTGACTCGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAG140330.1558285692521017No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT139450.15485137876580618No Hit
GTGATAAGTTGCAACATCCTCAGGTTGCAGGCTGGTGATGGTGAGAGTGA138400.15368541284465814No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA134810.1496989198380662No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT134400.14924363790695125No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG133180.14788889655095067No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC130530.14494622065471985No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC129940.14429105885140808No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT129720.1440467612298342No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC128540.14273643762321067No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG127690.14179256044894795No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA126100.14002695491120948No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG123850.13752845650874937No Hit
GATGAGGAGTTTAGGACCTGTCCCCGATTTCTCCTGATACCAGGCTATAT123300.13691771245481466No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA122310.1358183731577322No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGG121080.13445252736438734No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG120390.1336863211876329No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT120250.13353085906481316No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT120130.13339760581668197No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC118940.13207617777271416No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC115960.12876705544412254No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG115540.12830066907566332No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC114930.127623298397663No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA114110.12671273453543308No Hit
GTCCCAGATCCACTGCCACTGAATCGAGATGGGACCCCTGTTTCCAAAAT113260.12576885736117038No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA113160.1256578129877277No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA112860.1253246798673997No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT112710.1251581133072357No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA112330.12473614468815353No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAATCGAGATGGGACCCCT111790.12413650507156311No Hit
GATTTCACTCTCACCATCACCAGCCTGCAACCTGAGGATGTTGCAACTTA111240.12352576101762841No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAATCGAGA110840.12308158352385772No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC110740.12297053915041505No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC109910.12204887085084087No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCC109430.12151585785831603No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC109270.12133818686080777No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT108610.12060529399608613No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG108090.12002786325418424No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT106250.11798464678283907No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA105830.11751826041437985No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT105790.11747384266500278No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCT104650.11620793680775632No Hit
GTAATAGTTTTGACAGTGATAAGTTGCAACATCCTCAGGTTGCAGGCTGG104040.11553056612975603No Hit
TCCGTGCTGCATCCATTTTGGAAACAGGGGTCCCATCTCGATTCAGTGGC103500.11493092651316558No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC102110.11338740972231245No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100650.11176616187004942No Hit
ACTTATCACTGTCAAAACTATTACAGTGCCCCACTCACTTTCGGCGGAGG97370.10812390642112979No Hit
ACAGTGCCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA97290.10803507092237565No Hit
ATCGGGGACAGGTCCTAAACTCCTCATCCGTGCTGCATCCATTTTGGAAA97290.10803507092237565No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA96440.10709119374811293No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT96300.1069357316252932No Hit
ATATAATTGGCAATGCCCTGACTCGCCCGGCAAGTGATGGTGACTCTGTC94850.10532558821037445No Hit
GGTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAATCGAG93320.10362660929670157No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGT92950.10321574511496369No Hit
GGTTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC92600.10282708980791433No Hit
GCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACATCC92280.10247174781289779No Hit
GTCTCCGACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT92240.10242733006352071No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT91610.10172775051083187No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA91100.10116142420627426No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)