FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001963476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220086
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT140316.375235135356179No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61482.793453468189708No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT57742.623519896767627No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54302.467217360486355No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT30881.4030878838272312Illumina PCR Primer Index 10 (95% over 21bp)
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29441.3376589151513498No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT25411.1545486764264878Illumina PCR Primer Index 10 (95% over 23bp)
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19870.9028288941595557No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13970.6347518697236535No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12700.5770471542942304No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC7950.3612224312314277No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT6710.30488081931608557Illumina PCR Primer Index 10 (96% over 26bp)
TCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG6250.28397989876684565No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5850.2658051852457676No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT5840.26535081740774064No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG4950.2249120798233418Illumina PCR Primer Index 10 (96% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.2167334587388566No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT4750.21582472306280273No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG4070.1849277100769699Illumina PCR Primer Index 10 (95% over 24bp)
CAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.1822015030488082No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3680.16720736439391873No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC3680.16720736439391873No Hit
TCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.1658442608798379No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA3650.1658442608798379No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAACCTCGTATGCCGT3400.15448506492916406No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACACTCGCT3220.1463064438446789No Hit
ATCCAGTTCCAGCCAAGAGCAAGATGCCCAAAGTCTGAACTCACATTACT3000.13631035140808592No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGACTTTTTTTTTTTT2890.13131230518978945Clontech Universal Primer Mix Long (95% over 23bp)
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.12722299464754686No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAAACTCGTATGCCGT2780.12631425897149295No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2780.12631425897149295No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT2720.12358805194333125No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC2580.11722690221095389No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2470.11222885599265742No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC2460.11177448815463047No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAAC2420.10995701680252265No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2410.1095026489644957No Hit
GAACAAGGAAGTGAGGCTTCCCAAGAATTTAGCTTGCTGTGCAGTGGCTG2400.10904828112646874No Hit
GTTCCAGCCAAGAGCAAGATGCCCAAAGTCTGAACTCACATTACTGTCCA2360.10723080977436093No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAAGCTCGTATGCCGT2340.10632207409830702No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC2300.10450460274619922No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC2270.10314149923211836No Hit
AACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.10223276355606445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)