FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001963477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220086
Sequences flagged as poor quality0
Sequence length151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28961.3158492589260562No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28021.2731386821515227No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27461.2476940832220131No Hit
CTTATACACATCTGACGCTGCCGACGAATAGCCTTGTGTAGATCTCGGTG15310.6956371600192652No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14540.6606508364911898No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9280.4216533536890125No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7350.3339603609498105No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7060.32078369364702886No Hit
ATACACATCTGACGCTGCCGACGAATAGCCTTGTGTAGATCTCGGTGGTC4750.21582472306280273No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4510.20491989495015583No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.18947138845723943No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.18174713521078126No Hit
CATCTGACGCTGCCGACGAATAGCCTTGTGTAGATCTCGGTGGTCGCCGT3910.17765782466853866Illumina Single End PCR Primer 1 (100% over 23bp)
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA3530.16039184682351443No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT2750.1249511554574121No Hit
ATCCAGTTCCAGCCAAGAGCAAGATGCCCAAAGTCTGAACTCACATTACT2680.12177058059122342No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT2400.10904828112646874No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAAC2340.10632207409830702No Hit
GAACAAGGAAGTGAGGCTTCCCAAGAATTTAGCTTGCTGTGCAGTGGCTG2330.10586770626028008No Hit
GTTCCAGCCAAGAGCAAGATGCCCAAAGTCTGAACTCACATTACTGTCCA2280.10359586707014531No Hit
CAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.10177839571803748No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT2210.10041529220395663No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)