FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001963482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18304
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT250013.658216783216783No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT4802.6223776223776225Illumina PCR Primer Index 10 (95% over 22bp)
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4132.2563374125874125No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4062.2180944055944054No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT3862.1088286713286712Illumina PCR Primer Index 10 (95% over 24bp)
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3481.9012237762237765No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2121.1582167832167831No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG1430.78125No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.743006993006993No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC1070.5845716783216783No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC1030.5627185314685315No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.5080856643356643No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTACCTCGTATGCCGT670.3660402097902098No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.34418706293706297No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTG600.3277972027972028Illumina PCR Primer Index 10 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCT590.3223339160839161Illumina PCR Primer Index 10 (95% over 21bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCT580.3168706293706294Illumina PCR Primer Index 10 (96% over 27bp)
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT470.2567744755244755No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTG470.2567744755244755Illumina PCR Primer Index 10 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTAACTCGTATGCCGT410.22399475524475526No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTAGCTCGTATGCCGT360.1966783216783217No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGGAGCC350.19121503496503497No Hit
TCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.18028846153846154No Hit
TAGCTGTACTTTGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTCTTGA320.17482517482517482No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.15297202797202797No Hit
CAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.14204545454545456No Hit
GTATCAACGCAGAGTACGGGCTCCGAATACCGACGAGTGCGAGCAAGGGA250.13658216783216784No Hit
GCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG230.1256555944055944No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG220.1201923076923077No Hit
GGTATCAACGCAGAGTACGGGCTCCGAATACCGACGAGTGCGAGCAAGGG220.1201923076923077No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT200.10926573426573427No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC190.10380244755244755No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGACTTTTTTTTTTTT190.10380244755244755Clontech Universal Primer Mix Long (95% over 23bp)
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC190.10380244755244755No Hit
GTATCAACGCAGAGTACGGGGTAAAAGACTGGTTAATGATAACAATGCAT190.10380244755244755No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)