FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001963483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18304
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAATAGCCTTGTGTAGATCTCGGTG2821.5406468531468531No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.8850524475524476No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.704763986013986No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.6555944055944056No Hit
ATACACATCTGACGCTGCCGACGAATAGCCTTGTGTAGATCTCGGTGGTC970.5299388111888113No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.3933566433566434No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.2950174825174825No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.2076048951048951No Hit
CATCTGACGCTGCCGACGAATAGCCTTGTGTAGATCTCGGTGGTCGCCGT310.1693618881118881Illumina Single End PCR Primer 1 (100% over 23bp)
GGTATCAACGCAGAGTACGGGCTCCGAATACCGACGAGTGCGAGCAAGGG280.15297202797202797No Hit
GTATCAACGCAGAGTACGGGCTCCGAATACCGACGAGTGCGAGCAAGGGA280.15297202797202797No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC240.13111888111888112No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT220.1201923076923077No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC210.11472902097902098No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA210.11472902097902098No Hit
TAGCTGTACTTTGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTCTTGA190.10380244755244755No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.10380244755244755No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)