FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001963484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94394
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8560.9068372989808674No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8160.8644617242621354No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8150.863402334894167No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.47884399432167296No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT4350.46083437506621183No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.30722291671080787No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.2108184842256923No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC1540.16314596266711867No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.15255206898743565No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT1410.14937390088353072No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC1340.14195817530775262No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC1290.1366612284679111No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT1230.13030489226010128Illumina PCR Primer Index 11 (95% over 22bp)
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA1230.13030489226010128No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT1190.12606733478822807Illumina PCR Primer Index 11 (95% over 24bp)
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT1180.12500794542025978No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC1140.12077038794838656No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)