FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001963518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686111
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT86041.2540244945788654No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78451.1434009948827522No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70041.0208260762471377No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44610.6501863401111482No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT30700.44744946517400247No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26510.3863806293733813No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18430.2686154281158588No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13800.20113363581111512No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT10740.15653443830517222No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT7910.11528746806274785TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT7570.11033200167319865RNA PCR Primer, Index 7 (95% over 23bp)
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC6950.10129556296284421No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)