FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001963527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972422
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84590.8698898214972512No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83840.8621771206328116No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT58940.6061154519334199No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40170.4130922582993803No Hit
CTTATACACATCTGACGCTGCCGACGATAAGGCTCGTGTAGATCTCGGTG28520.2932883048717532No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28460.2926712888025981No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27870.28660396412257233No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18800.19333170166861713No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17090.17574674369769502No Hit
CATCTGACGCTGCCGACGATAAGGCTCGTGTAGATCTCGGTGGTCGCCGT12520.12875068643037693Illumina RNA PCR Primer (96% over 25bp)
ATACACATCTGACGCTGCCGACGATAAGGCTCGTGTAGATCTCGGTGGTC11910.12247768972729947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)