FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001963533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214553
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG267501.207918708651362No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG151400.6836594111768831No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146750.6626619457741585No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT130760.5904577582925312No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115430.5212338562229037No Hit
ATGCAACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAG112680.5088160003395719No Hit
GTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGAC94980.4288901642904911No Hit
CACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCA80980.3656719888844385No Hit
CACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGT73070.3299537197800188No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71470.3227287854478985No Hit
CGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGG65970.297893073681235No Hit
GTCCGACACAGCTGGCACCTGCTACTGACCCTGCTGGCCTGCCCACAGGC64960.2933323338840841No Hit
GGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGA56030.25300816914293767No Hit
GTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGC55330.249847260372635No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA54440.24582839065039308No Hit
AGATGCAACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAG51780.23381693732324313No Hit
GACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCG51210.23124305446742527No Hit
CCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTG50230.2268177821890016No Hit
ACCCTGTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTA49290.22257313326888092No Hit
CCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCG46790.21128417337494296No Hit
ACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCC46350.20929731643360988No Hit
GGGGAGACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGT45810.20685890109651922No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41980.1895642145390063No Hit
GCCCAACAGGGTCACCGAGAGGACCGTGGACAAGTCCACCGGTAAACCCA41810.18879656526621852No Hit
CAACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGG41150.1858162798542189No Hit
ACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGACCCTGC41010.18518409810015835No Hit
CCGAGAGGACCGTGGACAAGTCCACCGGTAAACCCACCCTGTACAACGTG37350.1686570608154332No Hit
GTGTCGGACATGACCAGGGACACGTTGTACAGGGTGGGTTTACCGGTGGA36710.16576708708258506No Hit
GTCCACCGGTAAACCCACCCTGTACAACGTGTCCCTGGTCATGTCCGACA36700.16572193124300932No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC35540.16048385385222208No Hit
GTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGACCCTGCTGGC33230.15005285491022344No Hit
GAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCA32710.14770475125228433No Hit
GGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGACACGTTGTA31910.14409228408622415No Hit
CTGTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTG31200.14088621947634578No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29630.13379675266295277No Hit
CCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGACA28740.12977788294071085No Hit
GTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGC28690.12955210374283208No Hit
GGTCATGTCCGACACAGCTGGCACCTGCTACTGACCCTGCTGGCCTGCCC28410.128287740234711No Hit
ACCGTGGACAAGTCCACCGGTAAACCCACCCTGTACAACGTGTCCCTGGT27400.1237270004375601No Hit
AACCCACCCTGTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACC27400.1237270004375601No Hit
CACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGT26900.12146920845877249No Hit
CATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCA26740.12074671502556046No Hit
CAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAG26240.11848892304677286No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25870.11681815698247006No Hit
CCGGTAAACCCACCCTGTACAACGTGTCCCTGGTCATGTCCGACACAGCT25400.11469583252240972No Hit
CGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGA24840.1121671055061676No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24340.10990931352738002No Hit
CAGCTGGCACCTGCTACTGACCCTGCTGGCCTGCCCACAGGCTCGGGGCG24250.10950291097119826No Hit
CTACTGACCCTGCTGGCCTGCCCACAGGCTCGGGGCGGCTGGCCGCTCTG23780.10738058651113792No Hit
GTATCAACGCAGAGTACGGGGTACAACGTGTCCCTGGTCATGTCCGACAC23310.10525826205107758No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT23250.10498732701362307No Hit
GGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACC23030.10399389854295653No Hit
GTCATGTCCGACACAGCTGGCACCTGCTACTGACCCTGCTGGCCTGCCCA22410.1011942364892599No Hit
GTGGACAAGTCCACCGGTAAACCCACCCTGTACAACGTGTCCCTGGTCAT22340.10087814561222964No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)